Anti-Wnt1 Antibody (A304883)

Name: Anti-Wnt1 Antibody
Brand: Antibodies.com
SKU: A304883
Price: 500.0 USD
Availability: InStock

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$500

Rabbit polyclonal antibody to Wnt1 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Wnt1 Antibody

Description

Rabbit polyclonal antibody to Wnt1.

Specificity

Band is detected at ~46 kDa due to post translational modifications.

Applications

WB, IHC

Dilutions

WB: 1:1,000, IHC: 1:50

Reactivity

Human

Immunogen

Synthetic peptide from the C-terminal of Human Wnt1 (aa. 270-370).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Peptide affinity purification.

Concentration

1 mg/ml

Molecular Weight

46 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BMND16, INT1, OI15, oncogene Int1, Proto oncogene protein Wnt 1, Proto-oncogene Int-1 homolog, Proto-oncogene Wnt-1, Wingless type MMTV integration site family member 1, wingless-type MMTV integration site family, member 1 (oncogene INT1), Wnt 1, WNT1_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-Wnt1 Antibody (A304883)

Western blot analysis of human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~41 kDa Wnt1 protein using Anti-Wnt1 Antibody (A304883) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK293T. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:5,000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~41 kDa. Other Band(s): Band is detected at ~46 kDa, due to post translational modifications.

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Figure 2: Immunohistochemistry - Anti-Wnt1 Antibody (A304883)

Immunohistochemistry analysis of human breast cancer, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-Wnt1 Antibody (A304883) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X.

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