Anti-XRCC1 Antibody (A11152)

Name: Anti-XRCC1 Antibody
Brand: Antibodies.com
SKU: A11152
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to XRCC1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-XRCC1 Antibody

Description

Rabbit polyclonal antibody to XRCC1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 480-633 of human XRCC1 (NP_006288.2).

Sequence

NGAEDSGDTEDELRRVAEQKEHRLPPGQEENGEDPYAGSTDENTDSEEHQEPPDLPVPELPDFFQGKHFFLYGEFPGDERRKLIRYVTAFNGELEDYMSDRVQFVITAQEWDPSFEEALMDNPSLAFVRPRWIYSCNEKQKLLPHQLYGVVPQA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

82 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DNA repair protein XRCC1, RCC, X ray repair complementing defective repair in chinese hamster, X ray Repair Complementing Defective Repair in Chinese Hamster Cells, X ray repair complementing defective repair in chinese hamster cells 1, X ray repair cross complementing 1, X ray repair cross complementing protein 1, X ray repair, complementing defective, repair in Chinese hamster, X-ray repair cross-complementing protein 1, XRCC 1, XRCC1_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-XRCC1 Antibody (A11152)

Western blot analysis of extracts of various cell lines, using Anti-XRCC1 Antibody (A11152) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-XRCC1 Antibody (A11152)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-XRCC1 Antibody (A11152) at a dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-XRCC1 Antibody (A11152)

Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-XRCC1 Antibody (A11152) at a dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-XRCC1 Antibody (A11152)

Immunofluorescence analysis of HeLa cells using Anti-XRCC1 Antibody (A11152) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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