Anti-YAP1 Antibody [ARC53469] (A309959)

Name: Anti-YAP1 Antibody [ARC53469]
Brand: Antibodies.com
SKU: A309959

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This product is discontinued

Anti-YAP1 Antibody [ARC53469] (A309959) has been discontinued and is no longer available.

View all Anti-YAP1 Antibodies.

Specifications

Name

Anti-YAP1 Antibody [ARC53469]

Description

Rabbit monoclonal [ARC53469] antibody to YAP1.

Applications

WB, IP, ChIP

Dilutions

WB: 1:2,000-1:4,000, IP: 1:50-1:200, CUT&Tag: 1⁶ cells/1µg, CHIP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human YAP1.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC53469

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

65 kDa Yes associated protein, 65 kDa Yes-associated protein, COB1, YAP, YAp 1, YAP 65, YAP1_HUMAN, YAP2, YAP65, yes -associated protein delta, Yes associated protein 1, Yes associated protein 1 65kDa, Yes associated protein 2, yes associated protein beta, YKI, Yorkie homolog

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Anti-YAP1 Antibody [ARC53469] (A309959)

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina from 10⁵ K562 cells with 1µg of Anti-YAP1 Antibody [ARC53469] (A309959), and Goat Anti-Rabbit IgG H&L Antibody. The CUT&Tag results indicate the enrichment pattern of YAP1 in representative gene loci (PTBP1), as shown in figure.

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Figure 2: Western Blot - Anti-YAP1 Antibody [ARC53469] (A309959)

Western blot analysis of extracts of C6, using Anti-YAP1 Antibody [ARC53469] (A309959) at 1:4,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Western Blot - Anti-YAP1 Antibody [ARC53469] (A309959)

Western blot analysis of extracts of Mouse heart, using Anti-YAP1 Antibody [ARC53469] (A309959) at 1:4,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 4: Western Blot - Anti-YAP1 Antibody [ARC53469] (A309959)

Western blot analysis of extracts from wild type(WT) and YAP1 knockout (KO) HeLa cells, using Anti-YAP1 Antibody [ARC53469] (A309959) at 1:4,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.