Anti-ZEB1 Antibody (A88558)

Name: Anti-ZEB1 Antibody
Brand: Antibodies.com
SKU: A88558

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This product is discontinued

Anti-ZEB1 Antibody (A88558) has been discontinued and is no longer available.

View all Anti-ZEB1 Antibodies.

Specifications

Name

Anti-ZEB1 Antibody

Description

Rabbit polyclonal antibody to ZEB1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ZEB1 (NP_110378.3).

Sequence

MADGPRCKRRKQANPRRNNVTNYNTVVETNSDSDDEDKLHIVEEESVTDAADCEGVPEDDLPTDQTVLPGRSSEREGNAKNCWEDDRKEGQEILGPEAQA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

180 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AREB 6, AREB6, BZP, Delta crystallin enhancer binding factor 1, DELTA EF1, FECD6, MGC133261, Negative regulator of IL 2, Negative regulator of IL2, NIL 2 A, NIL 2 A zinc finger protein, NIL 2A, NIL-2-A zinc finger protein, NIL2A, Posterior polymorphous corneal dystrophy 3, PPCD3, Represses interleukin 2 expression, TCF 8, TCF-8, TCF8, Transcription factor 8, Transcription factor 8 (represses interleukin 2 expression), ZEB, ZEB 1, ZEB1_HUMAN, ZFHEP, ZFHX 1A, ZFHX1A, Zinc finger E box binding homeobox 1, Zinc finger E-box-binding homeobox 1, Zinc finger homeodomain enhancer binding protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-ZEB1 Antibody (A88558)

Western blot analysis of extracts of various cells, using Anti-ZEB1 Antibody (A88558) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5min.

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Figure 2: Immunohistochemistry - Anti-ZEB1 Antibody (A88558)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-ZEB1 Antibody (A88558) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-ZEB1 Antibody (A88558)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-ZEB1 Antibody (A88558) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-ZEB1 Antibody (A88558)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-ZEB1 Antibody (A88558) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.