Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Tight junction protein 1, Tight junction protein ZO-1, Tight junction protein ZO1, TJP1, zo-1, Zo1, ZO1_HUMAN, Zona occludens 1, Zona occludens 1 protein, Zona occludens protein 1, Zonula occludens 1 protein, Zonula occludens protein 1
Figure 1: Western Blot - Anti-ZO1 tight junction protein Antibody (A8449)
Western blot analysis of HeLa, using Anti-ZO1 tight junction protein Antibody (A8449) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Figure 2: Immunofluorescence - Anti-ZO1 tight junction protein Antibody (A8449)
Immunofluorescence analysis of MCF7 cells using Anti-ZO1 tight junction protein Antibody (A8449) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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