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Anti-ZP3 Antibody (A90053)

Western Blot - Anti-ZP3 Antibody (A90053) - Antibodies.com Immunofluorescence - Anti-ZP3 Antibody (A90053) - Antibodies.com Immunofluorescence - Anti-ZP3 Antibody (A90053) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to ZP3 for WB and ICC/IF.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-ZP3 Antibody
Description
Rabbit polyclonal antibody to ZP3.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 250-350 of human ZP3 (NP_001103824.1).
Sequence
AFKVPRPGPDTLQFTVDVFHFANDSRNMIYITCHLKVTLAEQDPDELNKACSFSKPSNSWFPVEGSADICQCCNKGDCGTPSHSRRQPHVMSQWSRSASRN
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
47 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
HUMZP3, Processed zona pellucida sperm-binding protein 3, Sperm receptor, Zona pellucida glycoprotein 3, zona pellucida glycoprotein 3 (sperm receptor), zona pellucida glycoprotein 3A (sperm receptor), Zona pellucida glycoprotein ZP3, Zona pellucida protein C, Zona pellucida sperm binding protein 3 precursor, Zp-3, ZP3 372, ZP3 424, ZP3 474, ZP3A, ZP3A/ZP3B, ZP3B, ZP3_HUMAN, ZPC
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-ZP3 Antibody (A90053) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-ZP3 Antibody (A90053)
Western blot analysis of extracts of HeLa cells, using Anti-ZP3 Antibody (A90053) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Immunofluorescence - Anti-ZP3 Antibody (A90053) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-ZP3 Antibody (A90053)
Immunofluorescence analysis of rat oophoroma cells using Anti-ZP3 Antibody (A90053) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-ZP3 Antibody (A90053) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-ZP3 Antibody (A90053)
Immunofluorescence analysis of mouse oophoroma cells using Anti-ZP3 Antibody (A90053) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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