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Anti-AIRE Antibody (A90221)

Western Blot - Anti-AIRE Antibody (A14183) - Antibodies.com
This product is discontinued

Anti-AIRE Antibody (A90221) has been discontinued and is no longer available.

View all AIRE Antibodies.

  • Specifications

Name
Anti-AIRE Antibody
Description
Rabbit polyclonal antibody to AIRE.
Applications
WB, IHC
Dilutions
WB: 1:500-1:2000, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human AIRE (NP_000374.1).
Sequence
MATDAALRRLLRLHRTEIAVAVDSAFPLLHALADHDVVPEDKFQETLHLKEKEGCPQAFHALLSWLLTQDSTAILDFWRVLFKDYNLERYGRLQPILDSFPKDVDLSQPRKGRKPPAVPKALVPPPRLPTKRKASEEARAAAPAALTPRGTASPGSQLKAKPPKKPESSAEQQRLPLGNGIQTMSASVQRAVAMSSGDVPGARGAVEGILIQQVFESGGSKKCIQVGGEFYTPSKFEDSGSGKNKARSSSGPKPLVRAKGAQGAAPGGGEARLGQQGSVP
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
50kDa
Formulation
Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
APECED, APECED protein, Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein, Autoimmune regulator
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-AIRE Antibody (A14183) - Antibodies.comEnlarge Image

Western Blot - Anti-AIRE Antibody (A90221)

Western blot analysis of extracts of various cell lines, using Anti-AIRE Antibody (A14183) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

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Proteins predicted to interact with AIRE

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
86.9% Relevancy Score
83.9% Relevancy Score
80.3% Relevancy Score
77.2% Relevancy Score
76.6% Relevancy Score
76.4% Relevancy Score
75.6% Relevancy Score
74.6% Relevancy Score
73.3% Relevancy Score