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Anti-AKT (phospho-S473) Antibody

Total Citations (71)
Rabbit polyclonal antibody to AKT (phospho-S473) from Bioworld Technology (BS4007).
Name: Anti-AKT (phospho-S473) Antibody
Description: Rabbit polyclonal antibody to AKT (phospho-S473)
Specificity: p-AKT (S473) pAb detects endogenous levels of AKT1 only when phosphorylated at Ser473. This antibody also recognizes AKT2 and AKT3 when phosphorylated at the corresponding residues.
Applications: WB, IHC
Reactivity: Human, Mouse, Rat
Immunogen: Synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Serine 473.
Host: Rabbit
Clonality: Polyclonal
Conjugate: Unconjugated
Molecular Weight: ~ 60 kDa
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Product Form: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Function: AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity. Phosphorylation of GSK3 isoforms by AKT is also thought to be one mechanism by which cell proliferation is driven. AKT regulates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase). Phosphorylation of 'Ser-83' decreases MAP3K5 kinase activity stimulated by oxidative stress and thereby prevents apoptosis. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. AKT is involved in the phosphorylation of members of the FOXO factors (Forkhead family of transcription factors), leading to binding of 14-3-3 proteins and cytoplasmic localization. In particular, FOXO1 is phosphorylated at 'Thr-24', 'Ser-256' and 'Ser-319'. FOXO3 and FOXO4 are phosphorylated on equivalent sites. AKT has an important role in the regulation of NF-kappa-B-dependent gene transcription and positively regulates the activity of CREB1 (cyclic AMP (cAMP)-response element binding protein). The phosphorylation of CREB1 induces the binding of accessory proteins that are necessary for the transcription of pro-survival genes such as BCL2 and MCL1. AKT phosphorylates 'Ser-454' on ATP citrate lyase (ACLY), thereby potentially regulating ACLY activity and fatty acid synthesis. Activates the 3B isoform of cyclic nucleotide phosphodiesterase (PDE3B) via phosphorylation of 'Ser-273', resulting in reduced cyclic AMP levels and inhibition of lipolysis. Phosphorylates PIKFYVE on 'Ser-318', which results in increased PI(3)P-5 activity. The Rho GTPase-activating protein DLC1 is another substrate and its phosphorylation is implicated in the regulation cell proliferation and cell growth. AKT plays a role as key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). AKT mediates the antiapoptotic effects of IGF-I. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. May be involved in the regulation of the placental development. Phosphorylates STK4/MST1 at 'Thr-120' and 'Thr-387' leading to inhibition of its: kinase activity, nuclear translocation, autophosphorylation and ability to phosphorylate FOXO3. Phosphorylates STK3/MST2 at 'Thr-117' and 'Thr-384' leading to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation. Phosphorylates SRPK2 and enhances its kinase activity towards SRSF2 and ACIN1 and promotes its nuclear translocation. Phosphorylates RAF1 at 'Ser-259' and negatively regulates its activity. Phosphorylation of BAD stimulates its pro-apoptotic activity. Phosphorylates KAT6A at 'Thr-369' and this phosphorylation inhibits the interaction of KAT6A with PML and negatively regulates its acetylation activity towards p53/TP53.
Tissue Specificity: Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
Involvement in Disease: Breast cancer: A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.

Colorectal cancer: A complex disease characterized by malignant lesions arising from the inner wall of the large intestine (the colon) and the rectum. Genetic alterations are often associated with progression from premalignant lesion (adenoma) to invasive adenocarcinoma. Risk factors for cancer of the colon and rectum include colon polyps, long-standing ulcerative colitis, and genetic family history.

Proteus syndrome: A highly variable, severe disorder of asymmetric and disproportionate overgrowth of body parts, connective tissue nevi, epidermal nevi, dysregulated adipose tissue, and vascular malformations. Many features of Proteus syndrome overlap with other overgrowth syndromes.

Cowden syndrome 6: A form of Cowden syndrome, a hamartomatous polyposis syndrome with age-related penetrance. Cowden syndrome is characterized by hamartomatous lesions affecting derivatives of ectodermal, mesodermal and endodermal layers, macrocephaly, facial trichilemmomas (benign tumors of the hair follicle infundibulum), acral keratoses, papillomatous papules, and elevated risk for development of several types of malignancy, particularly breast carcinoma in women and thyroid carcinoma in both men and women. Colon cancer and renal cell carcinoma have also been reported. Hamartomas can be found in virtually every organ, but most commonly in the skin, gastrointestinal tract, breast and thyroid.
Sequence Similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Post-Translational Modification: O-GlcNAcylation at Thr-305 and Thr-312 inhibits activating phosphorylation at Thr-308 via disrupting the interaction between AKT1 and PDPK1. O-GlcNAcylation at Ser-473 also probably interferes with phosphorylation at this site.
Cellular Location: Cytoplasm. Nucleus. Cell membrane.

Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. Colocalizes with WDFY2 in intracellular vesicles (PubMed:16792529).
UniProt: P31749 /P31751 /Q9Y243
Synonyms: AKT 1 Antibody
AKT1 Antibody
AKT1_HUMAN Antibody
MGC99656 Antibody
PKB Antibody
PKB-ALPHA Antibody
PRKBA Antibody
Protein kinase B Antibody
Protein Kinase B Alpha Antibody
Proto-oncogene c-Akt Antibody
RAC Antibody
RAC Alpha Antibody
RAC-alpha serine/threonine-protein kinase Antibody
RAC-PK-alpha Antibody
Information: Target information shown above is from the UniProt Consortium.
For details please refer to datasheet.
For details please refer to datasheet.
For details please refer to datasheet.
For details please refer to datasheet.
For details please refer to datasheet.
Please Note: All batches are extensively validated and are guaranteed to work as specified, however, QC data is not necessarily from the current lot on sale.

The Study of Traditional Chinese Medical Elongated-needle Therapy promoting Neurological recovery mechanism after Spinal Cord Injury in Rats. J Ethnopharmacol (2016) Apr 13 :

miR-141 is involved in BRD7-mediated cell proliferation and tumor formation through suppression of the PTEN/AKT pathway in nasopharyngeal carcinoma. Cell Death Dis (2016) 7 : e2156

Sphingosine-1-phosphate receptor 2 mediates endothelial cells dysfunction by PI3K-Akt pathway under high glucose condition. Eur. J. Pharmacol. (2016) Apr 5 776 : 19-25

High Uric Acid Induces Insulin Resistance in Cardiomyocytes In Vitro and In Vivo. PLoS ONE (2016) 11 2: e0147737

The effects of Tao-Hong-Si-Wu on hepatic necroinflammatory activity and fibrosis in a murine model of chronic liver disease. J Ethnopharmacol (2016) Mar 2 180 : 28-36

Apelin-13 promotes cardiomyocyte hypertrophy via PI3K-Akt-ERK1/2-p70S6K and PI3K-induced autophagy. Acta Biochim. Biophys. Sin. (Shanghai) (2015) Dec 47 12: 969-80

Ethanolic Ginkgo biloba leaf extract prevents renal fibrosis through Akt/mTOR signaling in diabetic nephropathy. Phytomedicine (2015) Nov 15 22 12: 1071-8

Effects of lentivirus-mediated shRNA targeting integrin-linked kinase on oral squamous cell carcinoma in vitro and in vivo. Oncol. Rep. (2016) Jan 35 1: 89-98

Phosphocreatine protects endothelial cells from oxidized low-density lipoprotein-induced apoptosis by modulating the PI3K/Akt/eNOS pathway. Apoptosis (2015) Dec 20 12: 1563-76

Activation of mTOR signaling mediates the increased expression of AChE in high glucose condition: in vitro and in vivo evidences. Mol. Neurobiol. (2015) Sep 15 :

Gambogic acid enhances the radiosensitivity of human esophageal cancer cells by inducing reactive oxygen species via targeting Akt/mTOR pathway. Tumour Biol. (2016) Feb 37 2: 1853-62

Inhibition of ERR suppresses epithelial mesenchymal transition of triple negative breast cancer cells by directly targeting fibronectin. Oncotarget (2015) Sep 22 6 28: 25588-601

Fucoidan Suppresses Hypoxia-Induced Lymphangiogenesis and Lymphatic Metastasis in Mouse Hepatocarcinoma. Mar Drugs (2015) Jun 13 6: 3514-30

PM2.5-induced oxidative stress increases adhesion molecules expression in human endothelial cells through the ERK/AKT/NF-B-dependent pathway. J Appl Toxicol (2016) Jan 36 1: 48-59

Cordyceps sinensis polysaccharide inhibits PDGF-BB-induced inflammation and ROS production in human mesangial cells. Carbohydr Polym (2015) Jul 10 125 : 135-45

Decreased carboxylesterases expression and hydrolytic activity in type 2 diabetic mice through Akt/mTOR/HIF-1/Stra13 pathway. Xenobiotica (2015) 45 9: 782-93

Effect of baicalin-copper on the induction of apoptosis in human hepatoblastoma cancer HepG2 cells. Med. Oncol. (2015) Mar 32 3: 72

Frequent amplification of PTP1B is associated with poor survival of gastric cancer patients. Cell Cycle (2015) 14 5: 732-43

A novel Pseudolaric acid B derivative, Hexahydropseudolaric acid B, exterts an immunomodulatory effect in vitro/in vivo evaluation. Eur. J. Pharmacol. (2014) Dec 15 745 : 10-8

Ubiquitinspecific protease 22induced autophagy is correlated with poor prognosis of pancreatic cancer. Oncol. Rep. (2014) Dec 32 6: 2726-34

Angiogenin interacts with ribonuclease inhibitor regulating PI3K/AKT/mTOR signaling pathway in bladder cancer cells. Cell. Signal. (2014) Dec 26 12: 2782-92

The dipeptidyl peptidase-4 inhibitor sitagliptin protects against dyslipidemia-related kidney injury in Apolipoprotein E knockout mice. Int J Mol Sci (2014) 15 7: 11416-34

MiR-205 inhibits cell apoptosis by targeting phosphatase and tensin homolog deleted on chromosome ten in endometrial cancer Ishikawa cells. BMC Cancer (2014) 14 : 440

Berberine inhibits LPS-induced TF procoagulant activity and expression through NF-B/p65, Akt and MAPK pathway in THP-1 cells. Pharmacol Rep (2014) Jun 66 3: 480-4

Effect of Tenuifoliside A isolated from Polygala tenuifolia on the ERK and PI3K pathways in C6 glioma cells. Phytomedicine (2014) Sep 15 21 10: 1178-88

GPER mediates enhanced cell viability and motility via non-genomic signaling induced by 17-estradiol in triple-negative breast cancer cells. J. Steroid Biochem. Mol. Biol. (2014) Sep 143 : 392-403

Tripterygium glycosides impairs the proliferation of granulosa cells and decreases the reproductive outcomes in female rats. Birth Defects Res. B Dev. Reprod. Toxicol. (2014) Jun 101 3: 283-91

High uric acid directly inhibits insulin signalling and induces insulin resistance. Biochem. Biophys. Res. Commun. (2014) May 16 447 4: 707-14

Ribonuclease inhibitor up-regulation inhibits the growth and induces apoptosis in murine melanoma cells through repression of angiogenin and ILK/PI3K/AKT signaling pathway. Biochimie (2014) Aug 103 : 89-100

The DPP-4 inhibitor sitagliptin attenuates the progress of atherosclerosis in apolipoprotein-E-knockout mice via AMPK- and MAPK-dependent mechanisms. Cardiovasc Diabetol (2014) 13 : 32

Upregulation of estrogen receptor mediates migration, invasion and proliferation of endometrial carcinoma cells by regulating the PI3K/AKT/mTOR pathway. Oncol. Rep. (2014) Mar 31 3: 1175-82

MicroRNA-30b functions as a tumour suppressor in human colorectal cancer by targeting KRAS, PIK3CD and BCL2. J. Pathol. (2014) Mar 232 4: 415-27

Metallothionein 1G functions as a tumor suppressor in thyroid cancer through modulating the PI3K/Akt signaling pathway. BMC Cancer (2013) 13 : 462

Grape seed procyanidin reversal of p-glycoprotein associated multi-drug resistance via down-regulation of NF-B and MAPK/ERK mediated YB-1 activity in A2780/T cells. PLoS ONE (2013) 8 8: e71071

Cucurmosin induces the apoptosis of human pancreatic cancer CFPAC-1 cells by inactivating the PDGFR- signalling pathway. Pharmacol Rep (2013) 65 3: 682-8

The anti-apoptotic and neuro-protective effects of human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) on acute optic nerve injury is transient. Brain Res. (2013) Sep 26 1532 : 63-75

Activation of cannabinoid type 2 receptor by JWH133 protects heart against ischemia/reperfusion-induced apoptosis. Cell. Physiol. Biochem. (2013) 31 4-5: 693-702

Small interfering RNA targeting ILK inhibits metastasis in human tongue cancer cells through repression of epithelial-to-mesenchymal transition. Exp. Cell Res. (2013) Aug 1 319 13: 2058-72

-Catenin overexpression is associated with gefitinib resistance in non-small cell lung cancer cells. Pulm Pharmacol Ther (2014) Jun 28 1: 41-8

A novel role of ribonuclease inhibitor in regulation of epithelial-to-mesenchymal transition and ILK signaling pathway in bladder cancer cells. Cell Tissue Res. (2013) Sep 353 3: 409-23

Sprengerinin C exerts anti-tumorigenic effects in hepatocellular carcinoma via inhibition of proliferation and angiogenesis and induction of apoptosis. Eur. J. Pharmacol. (2013) Aug 15 714 1-3: 261-73

miR-145 inhibits isoproterenol-induced cardiomyocyte hypertrophy by targeting the expression and localization of GATA6. FEBS Lett. (2013) Jun 19 587 12: 1754-61

Effect of 5-hydroxymethylfurfural derived from processed Cornus officinalis on the prevention of high glucose-induced oxidative stress in human umbilical vein endothelial cells and its mechanism. Food Chem (2013) Sep 1 140 1-2: 273-9

5,7-Dihydroxyflavone Enhances the Apoptosis-Inducing Potential of TRAIL in Human Tumor Cells via Regulation of Apoptosis-Related Proteins. Evid Based Complement Alternat Med (2013) 2013 : 434709

Mechanisms underlying protective effects of trimetazidine on endothelial progenitor cells biological functions against H2O2-induced injury: involvement of antioxidation and Akt/eNOS signaling pathways. Eur. J. Pharmacol. (2013) May 5 707 1-3: 87-94

The expression of chitinase 3-like 1: a novel prognostic predictor for hepatocellular carcinoma. J. Cancer Res. Clin. Oncol. (2013) Jun 139 6: 1043-54

Dietary flavonoid genistein induces Nrf2 and phase II detoxification gene expression via ERKs and PKC pathways and protects against oxidative stress in Caco-2 cells. Mol Nutr Food Res (2013) Feb 57 2: 249-59

Combined treatment with an oncolytic adenovirus and antitumor activity of vincristine against retinoblastoma cells. Int J Mol Sci (2012) 13 9: 10736-49

Gleditsioside B, a triterpene saponin isolated from the anomalous fruits of Gleditsia sinensis Lam., abrogates bFGF-induced endothelial cell migration through preventing the activation of MMP-2 and FAK via inhibiting ERK and PI3K/AKT signaling pathways. Vascul. Pharmacol. (2013) Jan 58 1-2: 118-26

XJP-1 protects endothelial cells from oxidized low-density lipoprotein-induced apoptosis by inhibiting NADPH oxidase subunit expression and modulating the PI3K/Akt/eNOS pathway. Vascul. Pharmacol. (2013) Jan 58 1-2: 78-86

Expression patterns of USP22 and potential targets BMI-1, PTEN, p-AKT in non-small-cell lung cancer. Lung Cancer (2012) Sep 77 3: 593-9

Downregulation of integrin-linked kinase inhibits epithelial-to-mesenchymal transition and metastasis in bladder cancer cells. Cell. Signal. (2012) Jun 24 6: 1323-32

Glucose oxidase induces insulin resistance via influencing multiple targets invitro and invivo: The central role of oxidative stress. Biochimie (2012) Aug 94 8: 1705-17

Disruption of mindin exacerbates cardiac hypertrophy and fibrosis. J. Mol. Med. (2012) Aug 90 8: 895-910

Puerarin attenuates endothelial insulin resistance through inhibition of inflammatory response in an IKK/IRS-1-dependent manner. Biochimie (2012) May 94 5: 1143-50

Highly frequent PIK3CA amplification is associated with poor prognosis in gastric cancer. BMC Cancer (2012) 12 : 50

Does insulin bolster antioxidant defenses via the extracellular signal-regulated kinases-protein kinase B-nuclear factor erythroid 2 p45-related factor 2 pathway? Antioxid. Redox Signal. (2012) May 15 16 10: 1061-70

Anti-inflammatory and immunomodulatory mechanisms of artemisinin on contact hypersensitivity. Int. Immunopharmacol. (2012) Jan 12 1: 144-50

MiR-223 suppresses cell proliferation by targeting IGF-1R. PLoS ONE (2011) 6 11: e27008

Prevention of FGF-2-induced angiogenesis by scopoletin, a coumarin compound isolated from Erycibe obtusifolia Benth, and its mechanism of action. Int. Immunopharmacol. (2011) Dec 11 12: 2007-16

Monoclonal antibody to six transmembrane epithelial antigen of prostate-4 influences insulin sensitivity by attenuating phosphorylation of P13K (P85) and Akt: possible mitochondrial mechanism. J. Bioenerg. Biomembr. (2011) Jun 43 3: 247-55

Small interfering RNA targeting integrin-linked kinase inhibited the growth and induced apoptosis in human bladder cancer cells. Int. J. Biochem. Cell Biol. (2011) Sep 43 9: 1294-304

Isomalto oligosaccharide sulfate inhibits tumor growth and metastasis of hepatocellular carcinoma in nude mice. BMC Cancer (2011) 11 : 150

HOXB7 as a prognostic factor and mediator of colorectal cancer progression. Clin. Cancer Res. (2011) Jun 1 17 11: 3569-78

Effect of CIPC and intervention of Ca(2+)-regulated factors on CaN, cbl-b and p-AKT expression in neurons. Brain Res. (2011) May 10 1389 : 1-8

PI3K and ERK/Nrf2 pathways are involved in oleanolic acid-induced heme oxygenase-1 expression in rat vascular smooth muscle cells. J. Cell. Biochem. (2011) Jun 112 6: 1524-31

Modified Si-Miao-San extract inhibits inflammatory response and modulates insulin sensitivity in hepatocytes through an IKK/IRS-1/Akt-dependent pathway. J Ethnopharmacol (2011) Jul 14 136 3: 473-9

Luteolin inhibits inflammatory response and improves insulin sensitivity in the endothelium. Biochimie (2011) Mar 93 3: 506-12

Inhibition of vascular endothelial growth factor-induced angiogenesis by scopoletin through interrupting the autophosphorylation of VEGF receptor 2 and its downstream signaling pathways. Vascul. Pharmacol. (0) 54 1-2: 18-28

Ginsenoside Rg1 protects against hydrogen peroxide-induced cell death in PC12 cells via inhibiting NF-B activation. Neurochem. Int. (2011) Jan 58 1: 119-25

Lipoxin A(4) inhibits transition of epithelial to mesenchymal cells in proximal tubules. Am. J. Nephrol. (2010) 32 2: 122-36

Total Citations (43)
Description: Rabbit polyclonal antibody to AKT (A444)
Application: WB, IHC
Host: Rabbit
Reactivity: Human, Mouse, Rat
Immunogen: Synthetic peptide, corresponding to amino acids 420-470 of Human AKT1.

£165 – £283

Total Citations (34)
Description: Rabbit polyclonal antibody to AKT (phospho-T308)
Application: WB, IHC
Host: Rabbit
Reactivity: Human, Mouse, Rat
Immunogen: Synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Threonine 308.

£175 – £302

Total Citations (33)
Description: Rabbit polyclonal antibody to AKT
Application: WB, IHC
Host: Rabbit
Reactivity: Human, Mouse, Rat
Immunogen: Recombinant full length Human AKT1.

£165 – £283

Total Citations (18)
Description: Rabbit polyclonal antibody to Akt (Phospho-Ser473)
Application: WB, IHC
Host: Rabbit
Reactivity: Human, Mouse, Rat
Immunogen: Peptide sequence around phosphorylation site of serine 473 (Q-F-S(p)-Y-S) derived from Human Akt.

£180 – £234

Please Note: Anti-AKT (phospho-S473) Antibody is for research use only. It is not intended for diagnostic or therapeutic use.
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