Anti-Akt1 (phospho Ser473) Antibody [X20-A] (A8277)

Name: Anti-Akt1 (phospho Ser473) Antibody [X20-A]
Brand: Antibodies.com
SKU: A8277
Price: 535.0 USD
Availability: InStock

Enlarge Image

$535

Rabbit monoclonal (X20-A) antibody to Akt1 (phospho Ser473) for WB, ELISA, IP and ICC.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

4-6 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-Akt1 (phospho Ser473) Antibody [X20-A]

Description

Rabbit monoclonal (X20-A) antibody to Akt1 (phospho Ser473).

Applications

WB, ELISA, IP, ICC

Dilutions

WB: 1:5,000, ELISA: 1:100,000-1:200,000.

Reactivity

Human, Mouse, Rat

Immunogen

Peptide surrounding pSer-473 at the C-terminal sequence of human AKT1 protein.

Host

Rabbit

Clonality

Monoclonal

Clone ID

X20-A

Isotype

IgG

Conjugate

Unconjugated

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 8.0, with 10 mg/ml BSA and 0.05% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AKT, AKT 1, AKT1_HUMAN, MGC99656, PKB, PKB-ALPHA, PRKBA, Protein kinase B, Protein Kinase B Alpha, Proto-oncogene c-Akt, RAC, RAC Alpha, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

Enlarge Image

Figure 1: Western Blot - Anti-Akt1 (phospho Ser473) Antibody [X20-A] (A8277)

(A) Western blot analysis of Akt activation in striatal neurons stimulated with DHPG (mGluR5 agonist) for 0 (Lane 1), 2 (Lane 2), 5 (Lane 3) or 10 (Lane 4) minutes.

(B) Western blot analysis of total Akt, detected with Anti-Akt1 (phospho Ser473) Antibody (A8277) in striatal neurons stimulated with DHPG (a mGluR5 agonist) for 0 (Lane 1), 2 (Lane 2), 5 (Lane 3) or 10 (Lane 4) minutes. Wells were equally loaded with 100µg of whole cell lysate proteins.

Enlarge Image

Figure 2: Immunocytochemistry - Anti-Akt1 (phospho Ser473) Antibody [X20-A] (A8277)

Representative picture of Akt1 (only when phosphorylated at the Ser-473 residue) in HEK293 cells, visualized with Anti-Akt1 (phospho Ser473) Antibody (A8277). Primary antibody dilution: 1:300.

Publishing research using Anti-Akt1 (phospho Ser473) Antibody [X20-A] (A8277)? Please let us know so that we can list the citation on this page.