Anti-ALDH1L1 Antibody [2E7] (A85314)

Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with Anti-ALDH1L1 Antibody [2E7] (A85314), dilution 1:1,000, in red, and co-stained with Anti-GFAP Antibody (A85307), dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. The Anti-ALDH1L1 Antibody [2E7] produces cytoplasmic staining of glial cells, while the Anti-GFAP Antibody labels the intermediate filament cytoskeleton in astrocytes and other glial cells. Some astrocytic cells express both ALDH1L1 and GFAP and therefore appear yellow.

Western blot analysis of different tissue and cell lysates using Anti-ALDH1L1 Antibody [2E7] (A85314), dilution 1:5,000, in green. The lanes contain: [1] protein standard (red), rat tissue lysates: [2] heart, [3] liver, [4] kidney, [5] lung, [6] brain, and [7] spinal cord; mouse tissue lysates: [8] brain, and [9] spinal cord; cell lysates: [10] NIH-3T3, and [11] HEK293. The band at 100kDa mark corresponds to ALDH1L1 protein.