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Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody (A91218)

Western Blot - Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody (A91218) - Antibodies.com Immunohistochemistry - Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody (A91218) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Alkaline Phosphatase, Tissue Non-Specific for WB and IHC.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody
Description
Rabbit polyclonal antibody to Alkaline Phosphatase, Tissue Non-Specific.
Applications
WB, IHC
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 230-440 of human Alkaline Phosphatase (ALPL) (NP_000469.3).
Sequence
NKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNN
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
80 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
AKP2, Alkaline phosphatase liver/bone/kidney, Alkaline phosphatase liver/bone/kidney isozyme, Alkaline phosphatase tissue nonspecific isozyme, Alkaline phosphatase, tissue-nonspecific isozyme, Alkaline phosphomonoesterase, Alpl, AP TNAP, AP-TNAP, APTNAP, BAP, FLJ40094, FLJ93059, Glycerophosphatase, HOPS, Liver/bone/kidney type alkaline phosphatase, MGC161443, MGC167935, PHOA, PPBT_HUMAN, Tissue non specific alkaline phosphatase, Tissue nonspecific ALP, TNAP, TNSALP
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody (A91218) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody (A91218)
Western blot analysis of extracts of HeLa cells, using Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody (A91218) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry - Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody (A91218) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody (A91218)
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody (A91218) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Publishing research using Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody (A91218)? Please let us know so that we can list the citation on this page.

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