Anti-alpha 1 Fetoprotein Antibody [AMC0201] (A308568)

Name: Anti-alpha 1 Fetoprotein Antibody [AMC0201]
Brand: Antibodies.com
SKU: A308568

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This product is discontinued

Anti-alpha 1 Fetoprotein Antibody [AMC0201] (A308568) has been discontinued and is no longer available.

View all Anti-alpha 1 Fetoprotein Antibodies.

Highlighted products for Anti-alpha 1 Fetoprotein Antibody

A85533

12 Citations

Anti-alpha Fetoprotein Antibody [AFP-01]

Mouse monoclonal [AFP-01] antibody to alpha Fetoprotein for IP, ELISA, WB and ICC.

A85533

12 Citations

Anti-alpha Fetoprotein Antibody [AFP-01]

Mouse monoclonal [AFP-01] antibody to alpha Fetoprotein for IP, ELISA, WB and ICC.

Specifications

Name

Anti-alpha 1 Fetoprotein Antibody [AMC0201]

Description

Mouse monoclonal [AMC0201] antibody to alpha 1 Fetoprotein.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 19-609 of human Alpha-Fetoprotein (AFP).

Sequence

RTLHRNEYGIASILDSYQCTAEISLADLATIFFAQFVQEATYKEVSKMVKDALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSEEGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIARRHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSLLNQHACAVMKNFGTRTFQAITVTKLSQKFTKVNFTEIQKLVLDVAHVHEHCCRGDVLDCLQDGEKIMSYICSQQDTLSNKITECCKLTTLERGQCIIHAENDEKPEGLSPNLNRFLGDRDFNQFSSGEKNIFLASFVHEYSRRHPQLAVSVILRVAKGYQELLEKCFQTENPLECQDKGEEELQKYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQLSEDKLLACGEGAADIIIGHLCIRHEMTPVNPGVGQCCTSSYANRRPCFSSLVVDETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFSGLLEKCCQGQEQEVCFAEEGQKLISKTRAALGV

Host

Mouse

Clonality

Monoclonal

Clone ID

AMC0201

Isotype

IgG1

Light Chains

kappa

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

69 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Afp, AFPD, Alpha fetoglobulin, Alpha fetoprotein, Alpha fetoprotein precursor, Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein, alpha-fetoprotein, Hereditary persistence of, included, FETA, FETA_HUMAN, Hereditary persistence of alpha fetoprotein, HPAFP

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-alpha 1 Fetoprotein Antibody [AMC0201] (A308568)

Western blot analysis of extracts of HeLa cells, using Anti-alpha 1 Fetoprotein Antibody [AMC0201] (A308568) at 1:1,000 dilution. The secondary antibody was HRP Goat Anti-Mouse IgG (H+L) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-alpha 1 Fetoprotein Antibody [AMC0201] (A308568)

Immunohistochemistry analysis of paraffin-embedded mouse fetal liver using Anti-alpha 1 Fetoprotein Antibody [AMC0201] (A308568) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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