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Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100)

Western Blot - Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100) - Antibodies.com Immunohistochemistry - Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100) - Antibodies.com Immunofluorescence - Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100) - Antibodies.com Immunofluorescence - Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100) - Antibodies.com Immunofluorescence - Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC0298] antibody to alpha smooth muscle Actin for WB, IHC and ICC/IF.

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Highlighted products for Anti-alpha smooth muscle Actin Antibody

Western Blot - Anti-alpha smooth muscle Actin Antibody (A82445)
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A82445
1 Citation
Anti-alpha smooth muscle Actin Antibody
Goat polyclonal antibody to alpha smooth muscle Actin for ELISA and WB.
Immunohistochemistry - Anti-alpha Smooth Muscle Actin Antibody - Antibodies.com (A8234)
A8234
Anti-alpha Smooth Muscle Actin Antibody [M16-L]
Rabbit monoclonal (M16-L) antibody to alpha Smooth Muscle Actin for IHC-P and IHC-Fr.
Western Blot - Anti-alpha smooth muscle Actin Antibody (A82445)
(7)
A82445
1 Citation
Anti-alpha smooth muscle Actin Antibody
Goat polyclonal antibody to alpha smooth muscle Actin for ELISA and WB.
Immunohistochemistry - Anti-alpha Smooth Muscle Actin Antibody - Antibodies.com (A8234)
A8234
Anti-alpha Smooth Muscle Actin Antibody [M16-L]
Rabbit monoclonal (M16-L) antibody to alpha Smooth Muscle Actin for IHC-P and IHC-Fr.
Name
Anti-alpha smooth muscle Actin Antibody [ARC0298]
Description
Rabbit monoclonal [ARC0298] antibody to alpha smooth muscle Actin.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human a-Smooth Muscle Actin (ACTA2) (P62736).
Sequence
MCEEEDSTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAP
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC0298
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
42 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
a actin, AAT6, ACTA2, ACTA_HUMAN, Actin alpha 2 smooth muscle aorta, Actin aortic smooth muscle, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha 2 actin, Alpha actin 2, Alpha cardiac actin, alpha sma, Alpha-actin-2, Cell growth inhibiting gene 46 protein, Cell growth-inhibiting gene 46 protein, GIG46, Growth inhibiting gene 46, MYMY5
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100)
Western blot analysis of extracts of various cell lines, using Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry - Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100)
Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100)
Immunofluorescence analysis of smooth muscle of human colon carcinoma using Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100)
Immunofluorescence analysis of mouse colon using Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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