Anti-AMPK beta 1 Antibody [ARC1061] (A305692)

Name: Anti-AMPK beta 1 Antibody [ARC1061]
Brand: Antibodies.com
SKU: A305692
Price: 470.0 USD
Availability: InStock

Enlarge Image

$470

Rabbit monoclonal [ARC1061] antibody to AMPK beta 1 for WB, IHC and ICC/IF.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-AMPK beta 1 Antibody [ARC1061]

Description

Rabbit monoclonal [ARC1061] antibody to AMPK beta 1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human AMPKß1 (Q9Y478).

Sequence

EEIKAPEKEEFLAWQHDLEVNDKAPAQARPTVFRWTGGGKEVYLSGSFNNWSKLPLTRSHNNFVAILDLPEGEHQYKFFVDGQWTHDPSEPIVTSQLGTVN

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1061

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

40 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

1300015D22Rik, 5''-AMP-activated protein kinase subunit beta-1, 5'-AMP-activated protein kinase beta-1 subunit, AAKB1_HUMAN, AMP-activated protein kinase beta subunit, AMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA-1, AMP-activated, noncatalytic, beta-1, AMPK, AMPK beta 1 chain, AMPK subunit beta-1, AMPK-BETA-1, AMPKb, AU021155, E430008F22, HAMPKb, MGC17785, PRKAB1, Protein kinase AMP activated non catalytic subunit beta 1, protein kinase, AMP-activated, beta 1 non-catalytic subunit, protein kinase, AMP-activated, noncatalytic, beta-1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

Enlarge Image

Figure 1: Western Blot - Anti-AMPK beta 1 Antibody [ARC1061] (A305692)

Western blot analysis of extracts of various cell lines, using Anti-AMPK beta 1 Antibody [ARC1061] (A305692) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

Enlarge Image

Figure 2: Western Blot - Anti-AMPK beta 1 Antibody [ARC1061] (A305692)

Western blot analysis of extracts of Mouse brain, using Anti-AMPK beta 1 Antibody [ARC1061] (A305692) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-AMPK beta 1 Antibody [ARC1061] (A305692)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-AMPK beta 1 Antibody [ARC1061] (A305692) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Enlarge Image

Figure 4: Immunohistochemistry - Anti-AMPK beta 1 Antibody [ARC1061] (A305692)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-AMPK beta 1 Antibody [ARC1061] (A305692) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.