Anti-AMPK beta 1 (phospho Ser182) Antibody (A308809)

Name: Anti-AMPK beta 1 (phospho Ser182) Antibody
Brand: Antibodies.com
SKU: A308809
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to AMPK beta 1 (phospho Ser182) for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-AMPK beta 1 (phospho Ser182) Antibody

Description

Rabbit polyclonal antibody to AMPK beta 1 (phospho Ser182).

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S182 of human AMPKß1 (NP_006244.2).

Sequence

SSSPP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

38 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

1300015D22Rik, 5''-AMP-activated protein kinase subunit beta-1, 5'-AMP-activated protein kinase beta-1 subunit, AAKB1_HUMAN, AMP-activated protein kinase beta subunit, AMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA-1, AMP-activated, noncatalytic, beta-1, AMPK, AMPK beta 1 chain, AMPK subunit beta-1, AMPK-BETA-1, AMPKb, AU021155, E430008F22, HAMPKb, MGC17785, PRKAB1, Protein kinase AMP activated non catalytic subunit beta 1, protein kinase, AMP-activated, beta 1 non-catalytic subunit, protein kinase, AMP-activated, noncatalytic, beta-1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-AMPK beta 1 (phospho Ser182) Antibody (A308809)

Western blot analysis of extracts of C2C12 cells, using Anti-AMPK beta 1 (phospho Ser182) Antibody (A308809) at 1:1,000 dilution. C2C12 cells were treated by oligomycin (0. 5 uM) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-AMPK beta 1 (phospho Ser182) Antibody (A308809)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-AMPK beta 1 (phospho Ser182) Antibody (A308809) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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