Anti-ATF6 Antibody (A12536)

Name: Anti-ATF6 Antibody
Brand: Antibodies.com
SKU: A12536
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to ATF6 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-ATF6 Antibody

Description

Rabbit polyclonal antibody to ATF6.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:100-1:500, ICC/IF: 1:50-1:200, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human ATF6 (NP_031374.2).

Sequence

MGEPAGVAGTMESPFSPGLFHRLDEDWDSALFAELGYFTDTDELQLEAANETYENNFDNLDFDLDLMPWESDIWDINNQICTVKDIKAEPQPLSPASSSYSVSSPRSVDSYSSTQHVPEELDLSSSSQMSPLSLYGENSNSLSSAEPLKEDKPVTGPRNKTENGLTPKKKIQVNSKPSIQPKPLLLPAAP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

90 - 100 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Activating transcription factor 6, Activating transcription factor 6 alpha, ATF 6, ATF6 alpha, ATF6-alpha, ATF6A, ATF6A_HUMAN, cAMP dependent transcription factor ATF 6 alpha, cAMP-dependent transcription factor ATF-6 alpha, Cyclic AMP dependent transcription factor ATF 6 alpha, DKFZp686P2194, ESTM49, FLJ21663, Processed cyclic AMP dependent transcription factor ATF 6 alpha, Processed cyclic AMP-dependent transcription factor ATF-6 alpha

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-ATF6 Antibody (A12536)

Western blot analysis of various lysates, using Anti-ATF6 Antibody (A12536) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-ATF6 Antibody (A12536)

Western blot analysis of C6, using Anti-ATF6 Antibody (A12536) at 1:400 dilution. C6 cells were treated by tunicamycin (2 µg/ml) for 8 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Immunohistochemistry - Anti-ATF6 Antibody (A12536)

Immunohistochemistry analysis of paraffin-embedded human kidney using Anti-ATF6 Antibody (A12536) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-ATF6 Antibody (A12536)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-ATF6 Antibody (A12536) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.