Anti-ATG10 Antibody (A12112)

Name: Anti-ATG10 Antibody
Brand: Antibodies.com
SKU: A12112
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to ATG10 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-ATG10 Antibody

Description

Rabbit polyclonal antibody to ATG10.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 160 to the C-terminus of human ATG10 (NP_113670.1).

Sequence

FFVLHPCKTNEFMTPVLKNSQKINKNVNYITSWLSIVGPVVGLNLPLSYAKATSQDERNVP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

25 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Apg 10, APG 10L, Apg10, APG10 autophagy 10 like, APG10 like, APG10, S. cerevisiae, homolog of, APG10-like, APG10L, ATG 10, ATG10 autophagy related 10 homolog, ATG10 autophagy related 10 homolog (S. cerevisiae), ATG10_HUMAN, autophagy 10, S. cerevisiae, homolog of, Autophagy related protein 10, Autophagy-related protein 10, DKFZP586I0418, FLJ13954, pp12616, Ubiquitin-like-conjugating enzyme ATG10

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-ATG10 Antibody (A12112)

Western blot analysis of extracts of mouse small intestine, using Anti-ATG10 Antibody (A12112) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-ATG10 Antibody (A12112)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-ATG10 Antibody (A12112) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-ATG10 Antibody (A12112)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-ATG10 Antibody (A12112) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-ATG10 Antibody (A12112)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-ATG10 Antibody (A12112) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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