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Anti-ATG4A Antibody (A14097)

Western Blot - Anti-ATG4A Antibody (A14097) - Antibodies.com Immunofluorescence - Anti-ATG4A Antibody (A14097) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to ATG4A for WB and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-ATG4A Antibody
Description
Rabbit polyclonal antibody to ATG4A.
Applications
WB, ICC/IF
Dilutions
WB: 1:100-1:500, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 210-398 of human ATG4A (NP_443168.2).
Sequence
SKGTSAYCSAWKPLLLIVPLRLGINQINPVYVDAFKECFKMPQSLGALGGKPNNAYYFIGFLGDELIFLDPHTTQTFVDTEENGTVNDQTFHCLQSPQRMNILNLDPSVALGFFCKEEKDFDNWCSLVQKEILKENLRMFELVQKHPSHWPPFVPPAKPEVTTTGAEFIDSTEQLEEFDLEEDFEILSV
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
48 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
AI627006, APG4 autophagy 4 homolog A, Apg4a, ATG4 autophagy related 4 homolog A, ATG4 autophagy related 4 homolog A (S. cerevisiae), Atg4al, ATG4A_HUMAN, AUT like 2 cysteine endopeptidase, AUT-like 2 cysteine endopeptidase, Autl2, Autophagin 2, Autophagin-2, Autophagy related 4A cysteine peptidase, Autophagy related cysteine endopeptidase 2, Autophagy related protein 4 homolog A, Autophagy-related cysteine endopeptidase 2, Autophagy-related protein 4 homolog A, AV169859, Cysteine protease ATG4A, hAPG4A, MGC107179
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-ATG4A Antibody (A14097) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-ATG4A Antibody (A14097)
Western blot analysis of extracts of various cell lines, using Anti-ATG4A Antibody (A14097) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunofluorescence - Anti-ATG4A Antibody (A14097) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-ATG4A Antibody (A14097)
Immunofluorescence analysis of NIH/3T3 cells using Anti-ATG4A Antibody (A14097) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-ATG4A Antibody (A14097)? Please let us know so that we can list the citation on this page.

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