Anti-ATG4B Antibody [ARC1264] (A305633)

Name: Anti-ATG4B Antibody [ARC1264]
Brand: Antibodies.com
SKU: A305633

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This product is discontinued

Anti-ATG4B Antibody [ARC1264] (A305633) has been discontinued and is no longer available.

View all Anti-ATG4B Antibodies.

Specifications

Name

Anti-ATG4B Antibody [ARC1264]

Description

Rabbit monoclonal [ARC1264] antibody to ATG4B.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ATG4B (Q9Y4P1).

Sequence

DRHCNGFPAGAEVTNRPSPWRPLVLLIPLRLGLTDINEAYVETLKHCFMMPQSLGVIGGKPNSAHYFIGYVGEELIYLDPHTTQPAVEPTDGCFIPDESFH

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1264

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

48 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

APG4 autophagy 4 homolog B, APG4B, ATG4 autophagy related 4 homolog B (S. cerevisiae), ATG4B_HUMAN, AUT like 1 cysteine endopeptidase, AUT-like 1 cysteine endopeptidase, AUTL1, Autophagin 1, Autophagin-1, Autophagy related 4B cysteine peptidase, Autophagy related cysteine endopeptidase 1, Autophagy related protein 4 homolog B, Autophagy-related cysteine endopeptidase 1, Autophagy-related protein 4 homolog B, Cysteine protease ATG4B, hAPG4B, MGC1353

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-ATG4B Antibody [ARC1264] (A305633)

Western blot analysis of extracts of various cell lines, using Anti-ATG4B Antibody [ARC1264] (A305633) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunofluorescence - Anti-ATG4B Antibody [ARC1264] (A305633)

Immunofluorescence analysis of NIH-3T3 cells using Anti-ATG4B Antibody [ARC1264] (A305633) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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