Anti-AurB (phospho Thr232) Antibody (A93646)

Name: Anti-AurB (phospho Thr232) Antibody
Brand: Antibodies.com
SKU: A93646
Price: 185.0 USD
Availability: InStock

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$185

Rabbit polyclonal antibody to AurB (phospho Thr232) for WB, IHC, IF and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-AurB (phospho Thr232) Antibody

Description

Rabbit polyclonal antibody to AurB (phospho Thr232).

Specificity

This antibody detects endogenous levels of AurB only when phosphorylated at Thr232.

Applications

WB, IHC, IF, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, IF: 1:100-1:500, ELISA: 1:20000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human AurB around the phosphorylation site of Thr232 (amino acids 198-247).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

39kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AIE2, AIK3, AIM-1, AIRK3, ARK-2, ARK-3, ARK3, AURKB, AURKB_HUMAN, AURKC, Aurora 3, Aurora kinase B, Aurora kinase C, Aurora- and Ipl1-like midbody-associated protein 1, Aurora-related kinase 2, Aurora-related kinase 3, Aurora/IPL1-related kinase 2, Aurora/IPL1-related kinase 3, Aurora/IPL1/Eg2 protein 2, Serine/threonine-protein kinase 12, Serine/threonine-protein kinase 13, Serine/threonine-protein kinase aurora-B, Serine/threonine-protein kinase aurora-C, STK-1, STK13

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Immunofluorescence - Anti-AurB (phospho Thr232) Antibody (A93646)

Immunofluorescence analysis of HepG2 cells using Anti-AurB (phospho Thr232) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Figure 2: Immunohistochemistry - Anti-AurB (phospho Thr232) Antibody (A93646)

Immunohistochemical analysis of paraffin-embedded human liver carcinoma using Anti-AurB (phospho Thr232) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Figure 3: Western Blot - Anti-AurB (phospho Thr232) Antibody (A93646)

Western blot analysis of lysates from COS7 cells treated with Nocodazole 1µg/ml 16h using Anti-AurB (phospho Thr232) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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