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Anti-AurB (phospho Tyr12) Antibody (A94679)

Immunohistochemistry - Anti-AurB (phospho Tyr12) Antibody (A1133) - Antibodies.com Western Blot - Anti-AurB (phospho Tyr12) Antibody (A1133) - Antibodies.com
This product is discontinued

Anti-AurB (phospho Tyr12) Antibody (A94679) has been discontinued and is no longer available.

View all Aurora B Antibodies.

  • Specifications

Name
Anti-AurB (phospho Tyr12) Antibody
Description
Rabbit polyclonal antibody to AurB (phospho Tyr12).
Specificity
This antibody detects endogenous levels of AurB only when phosphorylated at Tyr12.
Applications
WB, IHC, ELISA
Dilutions
WB: 1:500~1:1000, IHC: 1:50~1:100, ELISA: 1:10000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human AurB around the phosphorylation site of Tyr12 (amino acids 1-50).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
39kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
AIK2, AIM-1, AIM1, AIRK2, ARK-2, ARK2, AURKB, Aurora 1, Aurora kinase B, Aurora- and IPL1-like midbody-associated protein 1, Aurora-related kinase 2, Aurora/IPL1-related kinase 2, Serine/threonine-protein kinase 12, STK-1, STK1, STK12, STK5
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Immunohistochemistry - Anti-AurB (phospho Tyr12) Antibody (A1133) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-AurB (phospho Tyr12) Antibody (A94679)

Immunohistochemical analysis of paraffin-embedded human liver carcinoma using Anti-AurB (phospho Tyr12) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Western Blot - Anti-AurB (phospho Tyr12) Antibody (A1133) - Antibodies.comEnlarge Image

Western Blot - Anti-AurB (phospho Tyr12) Antibody (A94679)

Western blot analysis of lysates from K562 cells using Anti-AurB (phospho Tyr12) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Proteins predicted to interact with Aurora B

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.8% Relevancy Score
99.7% Relevancy Score
99.7% Relevancy Score
99.7% Relevancy Score
99.6% Relevancy Score