Anti-Bcl-2 Antibody [8C8] (A249866)

Mouse monoclonal [8C8] antibody to Bcl-2 for Flow Cytometry, IF, WB and IHC-P.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Highlighted products for Anti-Bcl-2 Antibody

Name
Anti-Bcl-2 Antibody [8C8]
Description
Mouse monoclonal [8C8] antibody to Bcl-2.
Specificity
This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 lpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 lpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 lpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
Applications
Flow Cytometry, IF, WB, IHC-P
Dilutions
Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reactivity
Human, Monkey, Porcine
Cross Reactivity
This antibody does not cross react with Mouse and Rat.
Immunogen
Synthetic peptide corresponding to amino acids 41-54 of human Bcl-2 protein.
Sequence
GAAPAPGIFSSQPG-Cys
Host
Mouse
Clonality
Monoclonal
Clone ID
8C8
Isotype
IgG1
Light Chains
kappa
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
25-26 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Bcl-2 Antibody [8C8] - BSA and Azide free (A253046).
Synonyms
Apoptosis regulator Bcl 2, Apoptosis regulator Bcl-2, Apoptosis regulator Bcl2, AW986256, B cell CLL/lymphoma 2, B cell leukemia/lymphoma 2, Bcl2, BCL2_HUMAN, C430015F12Rik, D630044D05Rik, D830018M01Rik, Leukemia/lymphoma, B-cell, 2, Oncogene B-cell leukemia 2, PPP1R50, Protein phosphatase 1, regulatory subunit 50
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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