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Anti-Bcl-2 Antibody (A88945)

Western Blot - Anti-Bcl-2 Antibody (A88945) - Antibodies.com Western Blot - Anti-Bcl-2 Antibody (A88945) - Antibodies.com Immunohistochemistry - Anti-Bcl-2 Antibody (A88945) - Antibodies.com
This product is discontinued

Anti-Bcl-2 Antibody (A88945) has been discontinued and is no longer available.

View all Bcl-2 Antibodies.

  • Specifications

Name
Anti-Bcl-2 Antibody
Description
Rabbit polyclonal antibody to Bcl-2.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bcl-2 (NP_000624.2).
Sequence
MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAPAPGIFSSQPGHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQA
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
24 kDa / 26 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Apoptosis regulator Bcl-2, BCL2
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-Bcl-2 Antibody (A88945) - Antibodies.comEnlarge Image

Western Blot - Anti-Bcl-2 Antibody (A88945)

Western blot analysis of extracts of various cells, using Anti-Bcl-2 Antibody (A88945) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Western Blot - Anti-Bcl-2 Antibody (A88945) - Antibodies.comEnlarge Image

Western Blot - Anti-Bcl-2 Antibody (A88945)

Western blot analysis of extracts of HCT116 cells, using Anti-Bcl-2 Antibody (A88945) at 1:1,000 dilution. HCT116 cells treated with different concentrations of IL-8. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-Bcl-2 Antibody (A88945) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Bcl-2 Antibody (A88945)

Immunohistochemistry analysis of paraffin-embedded human stomach using Anti-Bcl-2 Antibody (A88945) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Proteins predicted to interact with Bcl-2

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score