Anti-Beclin 1 Antibody (A305169)

Name: Anti-Beclin 1 Antibody
Brand: Antibodies.com
SKU: A305169
Price: 500.0 USD
Availability: InStock

Enlarge Image

$500

Rabbit polyclonal antibody to Beclin 1 for WB and ICC/IF.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Tags:

Lysosome Marker

Specifications

Name

Anti-Beclin 1 Antibody

Description

Rabbit polyclonal antibody to Beclin 1.

Specificity

Predicted molecular weight at ~51 kDa.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Rat

Immunogen

Synthetic peptide from the C-terminus of human Beclin 1.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Peptide affinity purification.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

APG6, ATG 6, ATG6, ATG6 autophagy related 6 homolog, Bcl-2-interacting protein beclin, Beclin 1 (coiled coil moesin like BCL2 interacting protein), Beclin 1 autophagy related, Beclin-1, Beclin1, BECN 1, Becn1, BECN1_HUMAN, Coiled coil myosin like BCL2 interacting protein, Coiled-coil myosin-like BCL2-interacting protein, GT197, Protein GT197, VPS 30, VPS30

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

Enlarge Image

Figure 1: Western Blot - Anti-Beclin 1 Antibody (A305169)

Western blot analysis of human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~51kDa Beclin 1 protein using Anti-Beclin 1 Antibody (A305169) at 1:1,000 for 1 hour at room temperature. Lane 1: MW Ladder. Lane 2: human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at room temperature. The secondary antibody used was Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at room temperature. Color Development: TMB solution for 12 min at room temperature. Predicted/Observed Size: ~51kDa.

Enlarge Image

Figure 2: Western Blot - Anti-Beclin 1 Antibody (A305169)

Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of ~51kDa Beclin 1 protein using Anti-Beclin 1 Antibody (A305169) at 1:1,000 for 1 hour at room temperature. Lane 1: MW Ladder. Lane 2: human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at room temperature. The secondary antibody used was Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at room temperature. Color Development: TMB solution for 12 min at room temperature. Predicted/Observed Size: ~51kDa.

Enlarge Image

Figure 3: Immunofluorescence - Anti-Beclin 1 Antibody (A305169)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-Beclin 1 Antibody (A305169), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Golgi apparatus, Dendrites and Cell bodies of cerebellar Purkinje cells. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Beclin 1 Antibody (D) Composite.

Enlarge Image

Figure 4: Immunofluorescence - Anti-Beclin 1 Antibody (A305169)

Immunocytochemistry/Immunofluorescence analysis of human SK-N-BE, using Anti-Beclin 1 Antibody (A305169), at 1:200 for Overnight. The secondary antibody used was Anti-Rabbit: AlexaFluor 555 at 1:1,000. Counterstain: Hoechst, Phalloidin AlexaFluor 647 at 1:1,000. Localization: Mitochondria, endosomes/exosomes. A) Hoechst Nuclear Stain B) Phalloidin AlexaFluor 647 C) Anti-Beclin 1 D) Merge.

Publishing research using Anti-Beclin 1 Antibody (A305169)? Please let us know so that we can list the citation on this page.