Anti-Beclin 1 Antibody [ARC52858] (A307233)

Name: Anti-Beclin 1 Antibody [ARC52858]
Brand: Antibodies.com
SKU: A307233

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This product is discontinued

Anti-Beclin 1 Antibody [ARC52858] (A307233) has been discontinued and is no longer available.

View all Anti-Beclin 1 Antibodies.

Specifications

Name

Anti-Beclin 1 Antibody [ARC52858]

Description

Rabbit monoclonal [ARC52858] antibody to Beclin 1.

Applications

WB, ICC/IF

Dilutions

WB: 1:2,000-1:10,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Beclin 1 (NP_003757.1).

Sequence

MEGSKTSNNSTMQVSFVCQRCSQPLKLDTSFKILDRVTIQELTAPLLTTAQAKPGETQEEETNSGEEPFIETPRQDGVSRRFIPPARMMSTESANSFTLIGEASDGGTMENLSRRLKVTGDLFDIMSGQTDVDHPLCEECTDTLLDQLDTQLNVTENECQNYKRCLEILEQMNEDDSEQLQMELKELALEEERLIQELEDVEKNRKIVAENLEKVQAEAERLDQEEAQYQREYSEFKRQQLELDDELKSVENQMRYAQTQLDKLKKTNVFNATFHIWHSG

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC52858

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

APG6, ATG 6, ATG6, ATG6 autophagy related 6 homolog, Bcl-2-interacting protein beclin, Beclin 1 (coiled coil moesin like BCL2 interacting protein), Beclin 1 autophagy related, Beclin-1, Beclin1, BECN 1, Becn1, BECN1_HUMAN, Coiled coil myosin like BCL2 interacting protein, Coiled-coil myosin-like BCL2-interacting protein, GT197, Protein GT197, VPS 30, VPS30

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-Beclin 1 Antibody [ARC52858] (A307233)

Western blot analysis of various lysates, using Anti-Beclin 1 Antibody [ARC52858] (A307233) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-Beclin 1 Antibody [ARC52858] (A307233)

Western blot analysis of Rat lung, using Anti-Beclin 1 Antibody [ARC52858] (A307233) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunofluorescence - Anti-Beclin 1 Antibody [ARC52858] (A307233)

Immunofluorescence analysis of C6 using Anti-Beclin 1 Antibody [ARC52858] (A307233) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-Beclin 1 Antibody [ARC52858] (A307233)

Immunofluorescence analysis of HeLa using Anti-Beclin 1 Antibody [ARC52858] (A307233) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).