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Anti-beta Catenin Antibody [AMC0440] (A309681)

Western Blot - Anti-beta Catenin Antibody [AMC0440] (A309681) - Antibodies.com Immunohistochemistry - Anti-beta Catenin Antibody [AMC0440] (A309681) - Antibodies.com Immunohistochemistry - Anti-beta Catenin Antibody [AMC0440] (A309681) - Antibodies.com Immunohistochemistry - Anti-beta Catenin Antibody [AMC0440] (A309681) - Antibodies.com Immunohistochemistry - Anti-beta Catenin Antibody [AMC0440] (A309681) - Antibodies.com Immunohistochemistry - Anti-beta Catenin Antibody [AMC0440] (A309681) - Antibodies.com
$470
Product Datasheet

Mouse monoclonal [AMC0440] antibody to beta Catenin for WB and IHC.

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Mouse monoclonal [EM-22] antibody to beta Catenin for IP, WB, ICC and Flow Cytometry (Intracellular).
Name
Anti-beta Catenin Antibody [AMC0440]
Description
Mouse monoclonal [AMC0440] antibody to beta Catenin.
Applications
WB, IHC
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein of human ß-Catenin.
Host
Mouse
Clonality
Monoclonal
Clone ID
AMC0440
Isotype
IgG1
Light Chains
kappa
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
92 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Beta-catenin, Cadherin associated protein, Catenin (cadherin associated protein), beta 1, 88kDa, Catenin beta 1, Catenin beta-1, CATNB, CHBCAT, CTNB1_HUMAN, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, OTTHUMP00000162082, OTTHUMP00000165222, OTTHUMP00000165223, OTTHUMP00000209288, OTTHUMP00000209289
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-beta Catenin Antibody [AMC0440] (A309681) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-beta Catenin Antibody [AMC0440] (A309681)
Western blot analysis of extracts of various cell lines, using Anti-beta Catenin Antibody [AMC0440] (A309681) at 1:1,000 dilution. The secondary antibody was HRP Goat Anti-Mouse IgG (H+L) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-beta Catenin Antibody [AMC0440] (A309681) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-beta Catenin Antibody [AMC0440] (A309681)
Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-beta Catenin Antibody [AMC0440] (A309681) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 50 mM Tris/EDTA buffer pH 8.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-beta Catenin Antibody [AMC0440] (A309681) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-beta Catenin Antibody [AMC0440] (A309681)
Immunohistochemistry analysis of paraffin-embedded human hepatocellular carcinoma using Anti-beta Catenin Antibody [AMC0440] (A309681) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 50 mM Tris/EDTA buffer pH 8.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-beta Catenin Antibody [AMC0440] (A309681) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-beta Catenin Antibody [AMC0440] (A309681)
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-beta Catenin Antibody [AMC0440] (A309681) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 50 mM Tris/EDTA buffer pH 8.0 before commencing with IHC staining protocol.
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