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Anti-beta Catenin Antibody [ARC0136] (A307069)

Western Blot - Anti-beta Catenin Antibody [ARC0136] (A307069) - Antibodies.com Western Blot - Anti-beta Catenin Antibody [ARC0136] (A307069) - Antibodies.com Western Blot - Anti-beta Catenin Antibody [ARC0136] (A307069) - Antibodies.com Immunohistochemistry - Anti-beta Catenin Antibody [ARC0136] (A307069) - Antibodies.com Immunohistochemistry - Anti-beta Catenin Antibody [ARC0136] (A307069) - Antibodies.com Immunohistochemistry - Anti-beta Catenin Antibody [ARC0136] (A307069) - Antibodies.com Immunofluorescence - Anti-beta Catenin Antibody [ARC0136] (A307069) - Antibodies.com +1
Knockout Validated
$470
Product Datasheet

Rabbit monoclonal [ARC0136] antibody to beta Catenin for WB, IHC, ICC/IF and IP.

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Mouse monoclonal [EM-22] antibody to beta Catenin for IP, WB, ICC and Flow Cytometry (Intracellular).
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Anti-beta Catenin Antibody [EM-22]
Mouse monoclonal [EM-22] antibody to beta Catenin for IP, WB, ICC and Flow Cytometry (Intracellular).
Name
Anti-beta Catenin Antibody [ARC0136]
Description
Rabbit monoclonal [ARC0136] antibody to beta Catenin.
Applications
WB, IHC, ICC/IF, IP
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human beta Catenin (P35222).
Sequence
MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFP
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC0136
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
92 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Beta-catenin, Cadherin associated protein, Catenin (cadherin associated protein), beta 1, 88kDa, Catenin beta 1, Catenin beta-1, CATNB, CHBCAT, CTNB1_HUMAN, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, OTTHUMP00000162082, OTTHUMP00000165222, OTTHUMP00000165223, OTTHUMP00000209288, OTTHUMP00000209289
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-beta Catenin Antibody [ARC0136] (A307069) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-beta Catenin Antibody [ARC0136] (A307069)
Western blot analysis of extracts of HeLa cells, using Anti-beta Catenin Antibody [ARC0136] (A307069) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-beta Catenin Antibody [ARC0136] (A307069) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-beta Catenin Antibody [ARC0136] (A307069)
Western blot analysis of extracts of Mouse brain, using Anti-beta Catenin Antibody [ARC0136] (A307069) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-beta Catenin Antibody [ARC0136] (A307069) - Antibodies.comEnlarge Image
Figure 3: Western Blot - Anti-beta Catenin Antibody [ARC0136] (A307069)
Western blot analysis of extracts from wild type (WT) and beta-Catenin knockout (KO) 293T cells, using Anti-beta Catenin Antibody [ARC0136] (A307069) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry - Anti-beta Catenin Antibody [ARC0136] (A307069) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-beta Catenin Antibody [ARC0136] (A307069)
Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-beta Catenin Antibody [ARC0136] (A307069) at a dilution of 1:50(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Publishing research using Anti-beta Catenin Antibody [ARC0136] (A307069)? Please let us know so that we can list the citation on this page.

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