Anti-beta III Tubulin Antibody [AMC0115] (A308612)

Name: Anti-beta III Tubulin Antibody [AMC0115]
Brand: Antibodies.com
SKU: A308612

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This product is discontinued

Anti-beta III Tubulin Antibody [AMC0115] (A308612) has been discontinued and is no longer available.

View all Anti-beta III Tubulin Antibodies.

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Anti-beta III Tubulin Antibody [TU-20]

Mouse monoclonal [TU-20] antibody to beta III Tubulin for Flow Cytometry (Intracellular), WB, IHC-P and ICC.

(3)

A86691

67 Citations

Anti-beta III Tubulin Antibody [TU-20]

Mouse monoclonal [TU-20] antibody to beta III Tubulin for Flow Cytometry (Intracellular), WB, IHC-P and ICC.

Specifications

Name

Anti-beta III Tubulin Antibody [AMC0115]

Description

Mouse monoclonal [AMC0115] antibody to beta III Tubulin.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human TUBB3 (Q13509).

Sequence

SDLQLERISVYYNEASSHKYVPRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKLATPTYGDLNHLVSATMSGVTTSLRFPGQLNADLRKLAVNMVPF

Host

Mouse

Clonality

Monoclonal

Clone ID

AMC0115

Isotype

IgG2b

Light Chains

kappa

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

beta 3 tubulin, beta-4, CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, M(beta)3, M(beta)6, MC1R, Neuron specific beta III Tubulin, Neuron-specific class III beta-tubulin, QccE-11995, QccE-15186, TBB3_HUMAN, Tubb 3, TUBB3, TUBB4, Tubulin beta 3, Tubulin beta 3 chain, Tubulin beta 4, Tubulin beta III, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-beta III Tubulin Antibody [AMC0115] (A308612)

Western blot analysis of extracts from wild type(WT) and betaIII-Tubulin knockdown (KD) HeLa cells, using Anti-beta III Tubulin Antibody [AMC0115] (A308612) at 1:1,000 dilution. The secondary antibody was HRP Goat Anti-Mouse IgG (H+L) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-beta III Tubulin Antibody [AMC0115] (A308612)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-beta III Tubulin Antibody [AMC0115] (A308612) at dilution of 100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-beta III Tubulin Antibody [AMC0115] (A308612)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-beta III Tubulin Antibody [AMC0115] (A308612) at dilution of 100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-beta III Tubulin Antibody [AMC0115] (A308612)

Immunofluorescence analysis of mouse brain cells using Anti-beta III Tubulin Antibody [AMC0115] (A308612) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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