Anti-Bim Antibody (A88772)

Name: Anti-Bim Antibody
Brand: Antibodies.com
SKU: A88772
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Bim for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Bim Antibody

Description

Rabbit polyclonal antibody to Bim.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-198 of human Bim (NP_619527.1).

Sequence

DTDRSPAPMSCDKSTQTPSPPCQAFNHYLSAMASMRQAEPADMRPEIWIAQELRRIGDEFNAYYARRVFLNNYQAAEDHPRMVILRLLRYIVRLVWRMH

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

18 kDa / 23 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

B2L11_HUMAN, BAM, Bcl 2 interacting protein Bim, Bcl 2 related ovarian death agonist, Bcl-2-like protein 11, BCL2 interacting mediator of cell death, BCL2 like 11, BCL2 like 11 (apoptosis facilitator), BCL2 like protein 11, Bcl2-interacting mediator of cell death, Bcl2-L-11, Bcl2l11, BIM alpha6, BIM beta6, BIM beta7, BimEL, BimL, BOD

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Bim Antibody (A88772)

Western blot analysis of extracts of various cell lines, using Anti-Bim Antibody (A88772) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 2: Immunofluorescence - Anti-Bim Antibody (A88772)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Bim Antibody (A88772) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-Bim Antibody (A88772)

Immunofluorescence analysis of PC-12 cells using Anti-Bim Antibody (A88772) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-Bim Antibody (A88772)

Immunofluorescence analysis of U2OS cells using Anti-Bim Antibody (A88772) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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