Anti-C4d Antibody [10-11] (A278859)

Anti-C4d Antibody [10-11]

Mouse monoclonal [10-11] antibody to C4d.

This antibody recognises the secreted protein complement component 4d (C4d). The presence of C4d in renal peritubular capillaries is a key indicator for acute antibody-mediated rejection [AMR] (Collins et al. 1999.).C4d was accepted in 2003 into the Banff classification for identification of acute AMR. Mouse anti Human C4d antibody, clone 10-11 is specific for C4d, a marker that can be used in the detection of acute AMR for kidney, heart, pancreas and lung allografts. C4d is regarded as a key marker of antibody-mediated cell injury and humoral rejection. Complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4b’s alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonization and activation of the classical pathway. This requires C4 binding protein or CR1 as a cofactor. iC4b is further degraded into C4d and C4c. C4b’s short half life means that C4d is present in serum at high enough concentrations to make it a useful marker for classical complement activation. Mouse anti Human C4d antibody, clone 10-11 is used to detect the biomarker C4d which has been described as a “footprint” of antibody mediated tissue rejection. The internal thioester of C4b becomes exposed during cleavage to C4d and forms a covalent bond with the cell surface. The longer half-life of covalently bound C4d makes it a footprint of complement activation long after weakly bound antibodies have been cleared by the blood stream. C4 has also been linked to susceptibility to systemic lupus erythematosus and rheumatoid arthritis.

ELISA, WB, IHC-Fr, IHC-P, IF

ELISA: 1:5,000 - 1:20,000, IF: 1:250 - 1:600, IHC-Fr: 1:100 - 1:750

Human

This antibody does not cross react with Mouse, Dog, Bovine, Cat, Rabbit, Rat, Guinea Pig, or Sheep

Native C4d from human plasma.

Mouse

Monoclonal

10-11

IgG1

Unconjugated

Fast protein liquid chromatography (FPLC) of ascites.

Liquid

Supplied in Borate Buffered Saline with <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human C4d antibody, clone 10-11 recognizes the secreted protein complement component 4d (C4d). The presence of C4d in renal peritubular capillaries is a key indicator for acute antibody-mediated rejection [AMR] (Collins et al. 1999.).C4d was accepted in 2003 into the Banff classification for identification of acute AMR (Racusen et al. 2003). Mouse anti Human C4d antibody, clone 10-11 is specific for C4d, a marker that can be used in the detection of acute AMR for kidney, heart, pancreas and lung allografts. C4d is regarded as a key marker of antibody-mediated cell injury and humoral rejection (Sacks and Chowdhury 2002).Complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4b’s alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonization and activation of the classical pathway. This requires C4 binding protein or CR1 as a cofactor. iC4b is further degraded into C4d and C4c. C4b’s short half life means that C4d is present in serum at high enough concentrations to make it a useful marker for classical complement activation (Collins et al. 1999).Mouse anti Human C4d antibody, clone 10-11 is used to detect the biomarker C4d which has been described as a “footprint” of antibody mediated tissue rejection (Sacks and Chowdhury 2002). The internal thioester of C4b becomes exposed during cleavage to C4d and forms a covalent bond with the cell surface. The longer half-life of covalently bound C4d makes it a footprint of complement activation long after weakly bound antibodies have been cleared by the blood stream (Sacks and Chowdhury 2002).C4 has also been linked to susceptibility to systemic lupus erythematosus (Yang et al. 2004) and rheumatoid arthritis (Makinde et al. 1989).

acidic C4, Acidic complement C4, basic C4, basic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3, C4, C4, chido form, C4, Rodgers form, C4-1, C4A, C4a anaphylatoxin, C4A2, C4A3, C4A4, C4A6, C4AD, C4b, C4B1, C4B12, C4B2, C4B3, C4B5, C4BD, C4B_2, C4d fragment, C4F, C4S, CH, Chido form of C4, CO4, CO4A_HUMAN, Complement C4 gamma chain, complement C4-A, complement C4-B, complement C4-B-like, complement C4B1a, Complement component 4A, complement component 4A (Rodgers blood group), Complement component 4B, complement component 4B (Chido blood group), complement component 4B (Chido blood group), copy 2, complement component 4B (Chido blood group)-like, Complement component 4F, Complement component 4S, CPAMD2, CPAMD3, DADB-112B14.11, MGC164979, RG, Rodgers form of C4