Anti-Caldesmon Antibody (A250386)

Rabbit polyclonal antibody to Caldesmon for WB, Flow Cytometry, IF and IHC.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-Caldesmon Antibody
Description
Rabbit polyclonal antibody to Caldesmon.
Specificity
This antibody recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120 150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70 80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This antibody This antibody recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.
Applications
WB, Flow Cytometry, IF, IHC
Dilutions
WB: 1-2 µg/ml, Flow Cytometry: 0.5-1 µg/million cells, IF: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reactivity
Human, Rat
Immunogen
Recombinant fragment of human CALD1 protein.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
120-150 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Caldesmon Antibody - BSA and Azide free (A253566).
Synonyms
CAD, CALD 1, CALD1, CALD1_HUMAN, Caldesmon 1, Caldesmon 1 Isoform 1, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 5, Caldesmon1, CDM, H CAD, HCAD, L CAD, LCAD, MGC21352, NAG22
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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