Anti-Casein Kinase 2 beta Antibody [ARC1069] (A309247)

Name: Anti-Casein Kinase 2 beta Antibody [ARC1069]
Brand: Antibodies.com
SKU: A309247
Price: 470.0 USD
Availability: InStock

Enlarge Image

This product is discontinued

Anti-Casein Kinase 2 beta Antibody [ARC1069] (A309247) has been discontinued and is no longer available.

View all Anti-Casein Kinase 2 beta Antibodies.

Specifications

Name

Anti-Casein Kinase 2 beta Antibody [ARC1069]

Description

Rabbit monoclonal [ARC1069] antibody to Casein Kinase 2 beta.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 101-215 of human Casein Kinase 2 beta (CSNK2B) (CSNK2B) (P67870).

Sequence

YQQGDFGYCPRVYCENQPMLPIGLSDIPGEAMVKLYCPKCMDVYTPKSSRHHHTDGAYFGTGFPHMLFMVHPEYRPKRPANQFVPRLYGFKIHPMAYQLQLQAASNFKSPVKTIR

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1069

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

25 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Casein kinase 2 beta polypeptide, Casein kinase II beta subunit, Casein kinase II subunit beta, CK II beta, CK2B, CK2N, CSK2B, CSK2B_HUMAN, CSNK 2B, csnk2b, G5A, MGC138222, MGC138224, Phosvitin, Protein G5a

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

Enlarge Image

Figure 1: Western Blot - Anti-Casein Kinase 2 beta Antibody [ARC1069] (A309247)

Western blot analysis of extracts of various cell lines, using Anti-Casein Kinase 2 beta Antibody [ARC1069] (A309247) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

Enlarge Image

Figure 2: Immunohistochemistry - Anti-Casein Kinase 2 beta Antibody [ARC1069] (A309247)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Casein Kinase 2 beta Antibody [ARC1069] (A309247) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-Casein Kinase 2 beta Antibody [ARC1069] (A309247)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Casein Kinase 2 beta Antibody [ARC1069] (A309247) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Enlarge Image

Figure 4: Immunohistochemistry - Anti-Casein Kinase 2 beta Antibody [ARC1069] (A309247)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-Casein Kinase 2 beta Antibody [ARC1069] (A309247) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.