Anti-Caspase-4 Antibody (A9894)

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This product is discontinued

Anti-Caspase-4 Antibody (A9894) has been discontinued and is no longer available.

View all Caspase-4 Antibodies.

Specifications

Name

Anti-Caspase-4 Antibody

Description

Rabbit polyclonal antibody to Caspase-4.

Applications

Dilutions

WB: 1:100-1:500

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of mouse Caspase-4 (NP_031635.2).

Sequence

FLVLMSHGTLHGICGTMHSEKTPDVLQYDTIYQIFNNCHCPGLRDKPKVIIVQACRGGNSGEMWIRESSKPQLCRGVDLPRNMEADAVKLSHVEKDFIAFY

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

43 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CASP-4, CASP4, ICE and Ced-3 homolog 2, ICE(rel)-II, ICH-2, ICH2, Mih1, Protease TX

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-Caspase-4 Antibody (A9894)

Western blot analysis of extracts of HeLa cells, using Anti-Caspase-4 Antibody (A9894) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Western Blot - Anti-Caspase-4 Antibody (A9894)

Western blot analysis of extracts of Mouse lung, using Anti-Caspase-4 Antibody (A9894) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.