Anti-Caspase 3 (cleaved Asp175) Antibody (A94479)

Name: Anti-Caspase 3 (cleaved Asp175) Antibody
Brand: Antibodies.com
SKU: A94479
Price: 185.0 USD
Availability: InStock

Enlarge Image

$185

Rabbit polyclonal antibody to Caspase 3 (cleaved Asp175) for WB, IHC and ELISA.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-Caspase 3 (cleaved Asp175) Antibody

Description

Rabbit polyclonal antibody to Caspase 3 (cleaved Asp175).

Specificity

This antibody detects endogenous levels of fragment of activated Caspase 3 resulting from cleavage adjacent to Asp175.

Applications

WB, IHC, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:10000

Reactivity

Human

Immunogen

Synthetic peptide derived from human Caspase 3 (amino acids 157-206).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing peptide.

Molecular Weight

20 and 35kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CASP3, Caspase 3, Caspase 3 apoptosis related cysteine peptidase, CPP32, CPP32B, Procaspase 3, SCA 1, SCA1, Yama, Yama protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

Enlarge Image

Figure 1: Immunohistochemistry - Anti-Caspase 3 (cleaved Asp175) Antibody (A94479)

Immunohistochemical analysis of paraffin-embedded human brain tissue using Anti-Caspase 3 (cleaved Asp175) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

Enlarge Image

Figure 2: Western Blot - Anti-Caspase 3 (cleaved Asp175) Antibody (A94479)

Western blot analysis of lysates from HeLa cells, treated with Etoposide 25uM 60' using Anti-Caspase 3 (cleaved Asp175) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

Publishing research using Anti-Caspase 3 (cleaved Asp175) Antibody (A94479)? Please let us know so that we can list the citation on this page.