Anti-Caspase-9 Antibody (A12599)

Name: Anti-Caspase-9 Antibody
Brand: Antibodies.com
SKU: A12599
Price: 335.0 USD
Availability: InStock

Enlarge Image

$335

Rabbit polyclonal antibody to Caspase-9 for WB, IHC and ICC/IF.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-Caspase-9 Antibody

Description

Rabbit polyclonal antibody to Caspase-9.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 139-416 of Caspase-9 (NP_001220.2).

Sequence

VGALESLRGNADLAYILSMEPCGHCLIINNVNFCRESGLRTRTGSNIDCEKLRRRFSSLHFMVEVKGDLTAKKMVLALLELAQQDHGALDCCVVVILSHGCQASHLQFPGAVYGTDGCPVSVEKIVNIFNGTSCPSLGGKPKLFFIQACGGEQKDHGFEVASTSPEDESPGSNPEPDATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFPGFVSWRDPKSGSWYVETLDDIFEQWAHSEDLQSLLLRVANAVSVKGIYKQMPGCFNFLRKKLFFKTS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

35 kDa / 37 kDa / 47 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

APAF-3, APAF3, Apoptosis related cysteine peptidase, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, CASP9_HUMAN, Caspase 9 apoptosis related cysteine peptidase, Caspase 9 Dominant Negative, Caspase 9c, Caspase-9 subunit p10, ICE LAP6, ICE like apoptotic protease 6, ICE-LAP6, ICE-like apoptotic protease 6, MCH6, PPP1R56, protein phosphatase 1, regulatory subunit 56, RNCASP9

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

Enlarge Image

Figure 1: Western Blot - Anti-Caspase-9 Antibody (A12599)

Western blot analysis of extracts of various cell lines, using Anti-Caspase-9 Antibody (A12599) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Enlarge Image

Figure 2: Immunohistochemistry - Anti-Caspase-9 Antibody (A12599)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-Caspase-9 Antibody (A12599) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-Caspase-9 Antibody (A12599)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-Caspase-9 Antibody (A12599) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.