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Anti-Caspase-9 Antibody (A80515)

Western Blot - Anti-Caspase-9 Antibody (A80515) - Antibodies.com Western Blot - Anti-Caspase-9 Antibody (A80515) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Caspase-9 for WB.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-Caspase-9 Antibody
Description
Rabbit polyclonal antibody to Caspase-9.
Applications
WB
Dilutions
WB: 1:500-1:1,000
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 250-350 of human Caspase-9 (NP_001220.2).
Sequence
VYGTDGCPVSVEKIVNIFNGTSCPSLGGKPKLFFIQACGGEQKDHGFEVASTSPEDESPGSNPEPDATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFPG
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
47 kDa / 35 kDa / 37 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
APAF-3, APAF3, Apoptosis related cysteine peptidase, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, CASP9_HUMAN, Caspase 9 apoptosis related cysteine peptidase, Caspase 9 Dominant Negative, Caspase 9c, Caspase-9 subunit p10, ICE LAP6, ICE like apoptotic protease 6, ICE-LAP6, ICE-like apoptotic protease 6, MCH6, PPP1R56, protein phosphatase 1, regulatory subunit 56, RNCASP9
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Caspase-9 Antibody (A80515) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Caspase-9 Antibody (A80515)
Western blot analysis of extracts of various cell lines, using Anti-Caspase-9 Antibody (A80515) at 1:1,000 dilution. L929 cells were treated by staurosporine(1 uM) for 3 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western Blot - Anti-Caspase-9 Antibody (A80515) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Caspase-9 Antibody (A80515)
Western blot analysis of extracts of various cell lines, using Anti-Caspase-9 Antibody (A80515) at 1:1,000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37°C for 5 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
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