Human Cardiac Troponin I ELISA Kit (A105207)

Human Cardiac Troponin I ELISA Kit

Sandwich (quantitative)

This Cardiac Troponin I enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-Cardiac Troponin I Antibody. Standards or samples are then added to the microtire plate wells and Cardiac Troponin I, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of Cardiac Troponin I present in the sample, Anti-Cardiac Troponin I Antibody (HRP) is added to each well to "sandwich" the Cardiac Troponin I immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Cardiac Troponin I will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Microplate

Serum, cell culture supernatants, and tissue homogenates.

10 pg/ml

312-20,000 pg/ml

Human

Store at 4°C for 6 months or at -20°C for 12 months Store secondary antibody at 4°C.

TNNC1, TNNI3, Troponin I, cardiac muscle

Information online is representative. Always refer to the Product Manual inside the kit.