This Human Clusterin ELISA Kit applies a technique called quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for Clusterin. Standards or samples are then added to the appropriate microtiter plate wells and incubated. Clusterin if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove any unbound Clusterin and other components of the sample. A biotinylated Anti-Clusterin detection antibody is then added followed by Streptavidin-HRP. Addition of TMB substrate will result in a colored substrate product. The reaction is stopped with sulfuric acid and the optical density can be quantified using an ELISA plate reader. The concentration of analyte is determined by comparison to a serial dilution of the ELISA standard in parallel.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Serum, plasma, and cell culture supernatant.
Sensitivity
0.04 ng/ml
Product Range
0.07-50 ng/ml
Reactivity
Human
Storage
Shipped at 4°C. Store the standard at -20°C; avoid freeze / thaw cycles. Store all other reagents at 2-8°C.
Synonyms
Aging-associated gene 4 protein, Apo-J, APOJ, Apolipoprotein J, CLI, CLU, Complement cytolysis inhibitor, Complement-associated protein SP-40,40, Ku70-binding protein 1, KUB1
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
