Human Fas Ligand ELISA Kit (A341740)

Human Fas Ligand ELISA Kit

Human Fas Ligand ELISA kit includes pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB substrate, stop reagent, and a detailed assay procedure. The kit is designed for sensitive and quantitative detection of Human Fas Ligand in biological samples.

Sandwich (quantitative)

Human Fas Ligand ELISA Kit (A341740) is a sandwich ELISA designed for the sensitive and quantitative measurement of human Fas Ligand in serum, plasma and cell culture supernatants. The assay utilizes a 96-well microtiter plate pre-coated with an antibody specific for Fas Ligand. Standards and samples are added to the wells, allowing Fas Ligand present in the samples to bind to the immobilized capture antibody. After washing, a biotinylated detection antibody specific for Fas Ligand is added, forming an antibody–antigen–antibody complex. Unbound detection antibody is removed by washing, followed by the addition of streptavidin conjugated to horseradish peroxidase (HRP). Following incubation and washing, TMB substrate is added, resulting in a colorimetric reaction that produces a blue color which turns yellow upon addition of stop solution. The intensity of the color is directly proportional to the concentration of Fas Ligand in the sample. The concentration of Fas Ligand is determined by measuring the absorbance at 450 nm and comparing the results to the standard curve.

Pre-coated Microplate (1 x 96)

Colorimetric

Colorimetric Microplate Reader

12 pg/ml

62.5-2000 pg/ml

3h 45m

This product recognizesboth natural and recombinant soluble human Fas ligand.

Human

This product shows no cross reactivity with other human soluble molecules.

For bulk or custom IVD raw material requests, contact us directly.

IVD reagents are supplied in accordance with standard industry lead times of 4-6 weeks. Where products are in stock, dispatch typically occurs within 7-10 business days.

Store at +4°C.

Apoptosis antigen ligand, APT1LG1, APTL, CD178, CD95 ligand, CD95-L, CD95L, Fas antigen ligand, FasL, FASLG, TNFSF6, Tumor necrosis factor ligand superfamily member 6

Information online is representative. Always refer to the Product Manual inside the kit.