Human Fas Ligand ELISpot kit includes pre-coated PVDF plates, detection antibody, alkaline phosphatase conjugate, BSA, and BCIP/NBT ready-to-use substrate buffer. The kit is designed for sensitive, single-cell ELISpot detection of Human Fas Ligand secretion in PBMCs.
Principle of Assay
Human Fas Ligand ELISpot Kit (A341813) is a sandwich ELISA designed for the sensitive and quantitative measurement of human Fas Ligand in peripheral blood mononuclear cells (pbmcs). The assay utilizes a 96-well microtiter plate pre-coated with an antibody specific for Fas Ligand. Standards and samples are added to the wells, allowing Fas Ligand present in the samples to bind to the immobilized capture antibody. After washing, a biotinylated detection antibody specific for Fas Ligand is added, forming an antibody–antigen–antibody complex. Unbound detection antibody is removed by washing, followed by the addition of streptavidin conjugated to horseradish peroxidase (HRP). Following incubation and washing, TMB substrate is added, resulting in a colorimetric reaction that produces a blue color which turns yellow upon addition of stop solution. The intensity of the color is directly proportional to the concentration of Fas Ligand in the sample. The concentration of Fas Ligand is determined by measuring the absorbance at 450 nm and comparing the results to the standard curve.
Platform
Pre-coated Microplate (1 x 96)
Detection Type
Colorimetric
Sample Type
Peripheral blood mononuclear cells (PBMCs).
Assay Time
3h after cell stimulation
Specificity
This product recognizesnatural human Fas ligand.
Reactivity
Human
Cross Reactivity
This product shows no cross reactivity with other human soluble molecules.
General Notes
For bulk or custom IVD raw material requests, contact us directly. IVD reagents are supplied in accordance with standard industry lead times of 4-6 weeks. Where products are in stock, dispatch typically occurs within 7-10 business days.
