Human G-CSF Anti-Dug Assay ELISA Kit (A341472)

Human G-CSF Anti-Dug Assay ELISA Kit

Human G-CSF Anti-Dug Assay ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human G-CSF in serum and plasma.

Direct Sandwich (Quantitative)

Human G-CSF Anti-Drug Antibody ELISA Kit is an indirect ELISA that provides quantitative determination of antibodies targeting G-CSF in serum and plasma. G-CSF is coated onto the microplate to capture anti-GCSF antibodies present in samples. Enzyme-linked detection antibody and substrate addition produce a colorimetric signal proportional to antibody levels.

This kit contains: A coated 96 well microtiter plate, 1.8 mL Calibrator diluent, 12 µL Calibrator (1.15 mg/mL), 10X 50 mL wash buffer, 50 mL Assay buffer, 1000X 17 µL detection reagent, 12 mL TMB, 12 mL TMB stop solution.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric (HRP, OD450)

Serum and plasma.

45 ng/mL -1440 ng/mL

2.5 hours

15 ul

Human

Intra-assay CV: < 20%, Inter-assay CV: < 20%

Samples should be collected as serum or plasma using EDTA or heparin anticoagulants and stored at or below -20 °C for up to one year. Calibrators and test samples should be diluted 1:50 with assay buffer by adding 5 µL of sample to 245 µL of assay buffer. Mix thoroughly and do not store diluted samples.

Shipped at +4°C. Store the ELISA standard at -20°C. Store all other reagents at +4°C

C17orf33, CSF3, GCSF, Granulocyte colony-stimulating factor, Pluripoietin

Information online is representative. Always refer to the Product Manual inside the kit.