Human GM-CSF ELISA Kit (A334786)

Human GM-CSF ELISA Kit

Human GM-CSF ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human GM-CSF in serum, plasma, and cell culture supernatant.

Sandwich (quantitative)

Human GM-CSF ELISA Kit (A334786) employs a one-step sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of human GM-CSF in serum, plasma, and cell culture supernatant. A 96-well microtiter plate is provided pre-coated with an antibody specific for GM-CSF. Standards and test samples are added to the wells, allowing GM-CSF to bind to the immobilized capture antibody. A prepared antibody mixture is added and incubated to form antibody–antigen complexes. After incubation, unbound material is removed by washing, and TMB substrate is added to develop colour through an HRP-catalysed reaction. The reaction is stopped with acidic stop solution, producing a yellow endpoint signal. The intensity of the colour is proportional to the amount of GM-CSF captured in each well. The concentration of GM-CSF is determined by measuring absorbance at 450 nm and interpolating from the standard curve.

This ELISA Kit contains 10X 600 µL Capture Antibodies, 10X 600 µL, Detector Antibodies, 2 Lyophilized Recombinant Protein Vials, 50 mL Assay Diluent, 20X 1500 µL PTE Buffer, 10X 20 mL Wash Buffer, 12 mL TMB Solution, 12 mL Stop Solution, Pre-Coated 96-Well H-Microplate 12 x 8 well strips, 2 Plate Seals.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Colorimetric Microplate Reader

Serum, plasma, and cell culture supernatant.

3.125 pg/ml - 200 pg/ml

1 hour

Human

Cell culture supernatant: 88%; Serum: 94%; Plasma: 95%

Intra-assay: 4.2%, Inter-assay: 5.7%

The immunogens for the antibodies in this kit are recombinant GM-CSF.

Recombinant protein is supplied lyophilized; antibodies and buffer are supplied in liquid form.

Antibodies are supplied in Phosphate Buffered Saline with Tris-HCl.

This ELISA kit uses recombinant rabbit monoclonal antibodies.

Shipped at +4 °C. For short-term storage, keep at +4 °C. Shelf life is 12 months from the date of receipt or reconstitution.

Colony-stimulating factor, CSF, CSF2, GMCSF, Granulocyte-macrophage colony-stimulating factor, Molgramostin, Sargramostim

Information online is representative. Always refer to the Product Manual inside the kit.