Human Anti-Pentraxin 3 Antibody ELISA Kit (A335083)

Human Anti-Pentraxin 3 Antibody ELISA Kit

Human Anti-Pentraxin 3 Antibody ELISA Kit is a 3 hour indirect Enzyme-Linked Immunosorbent Assay (iELISA) designed for the in vitro quantitative determination of human Pentraxin 3 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.

Indirect (quantitative)

Human Anti-Pentraxin 3 Antibody ELISA Kit (A335083) is a 3 hour indirect Enzyme-Linked Immunosorbent Assay (iELISA) designed for the in vitro quantitative determination of human Pentraxin 3 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. A 96-well microtiter plate has been pre-coated with assay-specific antigen. Standards and samples are added to the wells, where Pentraxin 3 binds to the immobilized antigen. Following incubation, unbound components are removed by washing and an enzyme-conjugated detection antibody is added to the wells. This detection antibody binds the captured Pentraxin 3 to form an indirect immune complex. After further incubation and washing, TMB substrate solution is added to visualize the enzyme-mediated reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Pentraxin 3 present in the sample. The concentration of Pentraxin 3 is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric Detection (OD450)

Colorimetric Microplate Reader

Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.

0.938 ng/mL

3 hours

Human

Specific for Pentraxin 3 with no reported cross-reactivity with other analogs or variants.

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.

Pentaxin-related protein PTX3, Pentraxin-related protein PTX3, PTX3, TNF alpha-induced protein 5, TNFAIP5, TSG-14, TSG14, Tumor necrosis factor alpha-induced protein 5, Tumor necrosis factor-inducible gene 14 protein

Information online is representative. Always refer to the Product Manual inside the kit.