Human Pentraxin 3 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Pentraxin 3 in cell culture supernatant, serum and plasma.
Assay Type
Sandwich (quantitative)
Principle of Assay
Human Pentraxin 3 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Pentraxin 3 in the samples. A monoclonal antibody specific for human Pentraxin 3 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for Pentraxin 3, and Pentraxin 3 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Pentraxin 3 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
0.56 pg/ml
Product Range
31.25 - 2000 pg/ml
Specificity
This kit can assay both native and recombinant human Pentraxin 3.
Reactivity
Human
Cross Reactivity
This ELISA kit has shown no significant cross-reactivity.
Recovery
93% (Average Recovery from Serum Samples).
Storage
Store at +4°C. Do not use past expiration date!
Synonyms
Pentaxin-related protein PTX3, Pentraxin-related protein PTX3, PTX3, TNF alpha-induced protein 5, TNFAIP5, TSG-14, TSG14, Tumor necrosis factor alpha-induced protein 5, Tumor necrosis factor-inducible gene 14 protein
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
