Lyme Disease Borrelia burgdorferi sensu lato IgG + IgM ELISA Kit (A341255)

Lyme Disease Borrelia burgdorferi sensu lato IgG + IgM ELISA Kit

Lyme Disease Borrelia burgdorferi sensu lato IgG + IgM ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) designed for the in vitro quantitative determination of IgG and IgM antibodies to Borrelia burgdorferi sensu stricto, B. afzelii, B. garinii, B. mayonii, B. spielmanii, B. bissetiae, B. bavariensis, and B. valaisiana in serum and plasma.

Indirect ELISA

Lyme Disease Borrelia burgdorferi sensu lato IgG + IgM ELISA Kit employs an indirect ELISA for the quantitative measurement of IgG and IgM antibodies to Borrelia burgdorferi sensu stricto, B. afzelii, B. garinii, B. mayonii, B. spielmanii, B. bissetiae, B. bavariensis, and B. valaisiana in serum and plasma. The 96-well microplate is pre-coated with Borrelia antigens. Samples and standards are added and incubated, allowing specific antibodies to bind to the immobilized antigens. After washing, a biotinylated detection antibody is applied, followed by HRP-streptavidin. TMB substrate is added to generate a colourimetric reaction catalyzed by HRP. The reaction is stopped with acidic solution, and absorbance is read at 450 nm. The signal intensity is proportional to the concentration of Borrelia-specific antibodies present and is determined by comparison with a standard curve.

This ELISA kit includes a 96-well microplate pre-coated with proprietary Epitogen VlsE antigens and supplied ready for use. The kit also contains a peroxidase-labelled anti-human IgM antibody, 10× wash buffer, secondary diluent, TMB substrate and protective plate seals.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric Microplate Reader

Serum and plasma.

1h 40m

B. burgdorferi sensu stricto, B. afzelii, B. garinii, B. mayonii. B. spielmanii. B. bissetiae, B. bavariensis and B. valaisiana

This kit uses a defined panel of 27 VlsE antigens corresponding to the main Borrelia species and subspecies as the immunogen, enabling specific detection of VlsE-directed IgM antibodies with enhanced diagnostic specificity.

IgM

Shipped at 4°C. Store at 4°C.

Ig mu chain C region, Ig mu chain C region BOT, Ig mu chain C region GAL, Ig mu chain C region OU, IGHM, Immunoglobulin heavy constant mu

Information online is representative. Always refer to the Product Manual inside the kit.