Lyme Disease Borrelia burgdorferi sensu lato IgM ELISA Kit (A341254)

Lyme Disease Borrelia burgdorferi sensu lato IgM ELISA Kit

Lyme Disease Borrelia burgdorferi sensu lato IgM ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) designed for the in vitro quantitative determination of IgM antibodies to Borrelia burgdorferi sensu stricto, B. afzelii, B. garinii, B. mayonii, B. spielmanii, B. bissetiae, B. bavariensis, and B. valaisiana in serum and plasma.

Indirect ELISA

Lyme Disease Borrelia burgdorferi sensu lato IgM ELISA Kit employs an indirect ELISA for the quantitative measurement of IgM antibodies to Borrelia burgdorferi sensu stricto, B. afzelii, B. garinii, B. mayonii, B. spielmanii, B. bissetiae, B. bavariensis, and B. valaisiana in serum and plasma. The microplate wells are pre-coated with Borrelia antigens. Samples and standards are incubated in the wells to allow specific IgM antibodies to bind. After washing, a biotinylated detection antibody is added, followed by HRP-streptavidin. TMB substrate is then introduced, producing a colour change through HRP-mediated enzymatic activity. The reaction is stopped with acidic solution, and absorbance is measured at 450 nm. The optical density is directly proportional to the concentration of Borrelia-specific IgM in the sample and is quantified using a standard curve.

This ELISA kit includes a 96-well microplate pre-coated with proprietary Epitogen Lyme antigens and supplied ready for use. The kit also contains a peroxidase-labelled anti-human IgM antibody, 10× wash buffer, secondary diluent, TMB substrate and protective plate seals.

Pre-coated Microplate (12 x 8 well strips)

HRP Colorimetric

Serum and plasma.

The assay shows a sensitivity of 76.7 percent in late Lyme disease and Lyme neuroborreliosis, 45 percent in Lyme arthritis, and 57.1 percent in Lyme carditis, and demonstrates greater than 87.8 percent sensitivity in acute Lyme disease within six weeks of infection, with observed sensitivities of 55.1 percent in acute Lyme disease and 59.6 percent in early Lyme disease.

1h 40m

B. burgdorferi sensu stricto, B. afzelii, B. garinii, B. mayonii. B. spielmanii. B. bissetiae, B. bavariensis and B. valaisiana

This kit uses an immunogen composed of a mixture of 120 linear immunodominant epitopes together with 4 flagellin subunits, 12 full-length VlsE proteins, and 8 full-length OspC antigens, to capture IgM antibodies indicative of an early immune response to Borrelia.

IgM

Shipped at 4°C. Store at 4°C.

Ig mu chain C region, Ig mu chain C region BOT, Ig mu chain C region GAL, Ig mu chain C region OU, IGHM, Immunoglobulin heavy constant mu

Information online is representative. Always refer to the Product Manual inside the kit.