Mouse IRF6 ELISA Kit (A335171)

Mouse IRF6 ELISA Kit

Mouse IRF6 ELISA Kit is a 120 minute competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of mouse IRF6 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.

Competitive (quantitative)

Mouse IRF6 ELISA Kit (A335171) is a 120 minute competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of mouse IRF6 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for IRF6. During the assay, IRF6 present in the standards or samples competes with a fixed amount of assay-specific detection reagent for binding to the immobilized antibody. Following incubation, unbound components are removed by washing and HRP-conjugated detection reagent is added to the wells. After further incubation and washing, TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the colour is measured at 450 nm using a microplate reader. The concentration of IRF6 in the samples is inversely proportional to the OD450 value and is determined by comparison with the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric Detection (OD450)

Colorimetric Microplate Reader

Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.

0.188 µg/mL

2 hours

Mouse

Specific for IRF6 with no reported cross-reactivity with other analogs or variants.

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.

Interferon regulatory factor 6, IRF-6

Information online is representative. Always refer to the Product Manual inside the kit.