Principle of Assay
Rat EGF ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of rat EGF in the samples. A monoclonal antibody specific for rat EGF has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for EGF, and EGF present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of EGF bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
