Anti-Acrolein Antibody [10A10] (A304753)

Name: Anti-Acrolein Antibody [10A10]
Brand: Antibodies.com
SKU: A304753
Price: 475.0 GBP
Availability: InStock

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$630

Mouse monoclonal [10A10] antibody to Acrolein for WB, ICC/IF, FACS, Flow Cytometry, ELISA and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-Acrolein Antibody [10A10]

Description

Mouse monoclonal [10A10] antibody to Acrolein.

Specificity

This antibody is specific to Acrolein modified proteins.

Applications

WB, ICC/IF, FACS, Flow Cytometry, ELISA, IHC

Dilutions

WB: 1:1,000, ICC/IF: 1:50, FACS: 1:50, FCM: 1:50, ELISA: 1:1,000, IHC: 1:100

Reactivity

Species Independent

Cross Reactivity

This antibody does not cross-react with Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.

Immunogen

Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH)

Host

Mouse

Clonality

Monoclonal

Clone ID

10A10

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Flow Cytometry - Anti-Acrolein Antibody [10A10] (A304753)

Flow cytometry analysis of human neuroblastoma cells (SH-SY5Y), fixed in 90% methanol, using Anti-Acrolein Antibody [10A10] (A304753) at 1:50 for 30 minutes on ice. The secondary antibody used was Goat Anti-Mouse: PE at 1:100 for 20 minutes at room temperature. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.

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Western Blot - Anti-Acrolein Antibody [10A10] (A304753)

Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of Acrolein protein using Anti-Acrolein Antibody [10A10] (A304753) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 µg. Block: 5% Skim Milk in TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes in room temperature.

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Western Blot - Anti-Acrolein Antibody [10A10] (A304753)

Western blot analysis of Acrolein-BSA Conjugate showing detection of 67 kDa Acrolein protein using Anti-Acrolein Antibody [10A10] (A304753) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: AcroleinBSA (0.5 µg). Lane 3: AcroleinBSA (2.0 µg). Lane 4: BSA (0.5 µg). Lane 5: BSA (2.0 µg). Block: 5% Skim Milk in TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes in room temperature. Predicted/Observed Size: 67 kDa.

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Immunofluorescence - Anti-Acrolein Antibody [10A10] (A304753)

Immunocytochemistry/Immunofluorescence analysis of human embryonic kidney epithelial cell line (HEK293), fixed in 5% formaldehyde for 5 minutes, using Anti-Acrolein Antibody [10A10] (A304753), at 1:50 for 30-60 min at room temperature. The secondary antibody used was Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at room temperature. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50,000 for 30-60 min at room temperature. Magnification: 20X (2X Zoom). (A,C,E,G) Untreated. (B,D,F,H) Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) Acrolein Antibody. (G,H) Composite.