Rabbit polyclonal antibody to ADD1 (Phospho-Ser726)
Specificity
The antibody detects endogenous level of ADD1only when phosphorylated at serine 726.
Applications
WB, IHC, IF
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 726 (T-P-S(p)-F-L) derived from Human ADD1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Western blot analysis of extracts from Hela cells using ADD1 (Phospho-Ser726) Antibody #11182 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ADD1 (Phospho-Ser726) Antibody #11182 (left) or the same antibody preincubated with blocking peptide (right).
